Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof

A technology of cucumber wilt and Trichoderma, applied in the field of microorganisms, to achieve the effects of fast growth, large spore production, and enhanced specialization

Inactive Publication Date: 2013-01-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof
  • Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof
  • Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof

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Embodiment

[0018] 1. Acquisition of Trichoderma harzianum SH2303 CGMCC NO.4963

[0019] For 1032 wild strains of Trichoderma isolated from the soil of vegetable protection land in East China, the applicant selected the growth rate of Trichoderma, the amount of sporulation and the inhibition rate of pathogenic bacteria in vitro for preliminary screening, and then passed the non-volatile substances and volatiles. Trichoderma harzianum (Trichoderma harzianum) SH2303 CGMCC NO. 4963.

[0020] The specific process is as follows: Collect soil from vegetable protection land in East China→soil preservation at 4°C→isolate and purify strains (a total of 1032 strains)→primary screening indicators (growth speed, spore production and pathogenic bacteria inhibition rate against in vitro confrontation)→rescreening (Detection of non-volatile substances and volatile substances, detection of chitinase activity)→In vivo inoculation control effect test. After repeated screening through the above steps, a T...

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Abstract

The invention relates to a trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof. The trichoderma strain is Trichoderma harzianum SH2303 CGMCC NO. 4963, and has the capacity of antagonizing cucumber fusarium wilt disease. The invention also relates to application of the strain to the preparation of pesticides for preventing and controlling cucumber fusarium wilt. The trichoderma strain is high in growing speed and large in spore-bearing quantity, the inhibition rate of in vitro on pathogenic bacteria is 43.01 percent, the inhibition rate of non-volatile inhibitors on cucumber fusarium wilt is 41.33 percent, the inhibition rate of volatile substances on cucumber fusarium wilt is 11.49 percent, the enzyme activity of chitin is 3.9573U, the enzyme activity of beta-1 and 3-glucanase is 0.5092U, the enzyme activity of extracellular protease is 3.0678U; and the in-vivo prevention effect of cucumber fusarium wilt is 71.43 percent, the total specific gravity of antibiotic secondary metabolites which achieve an antagonistic action in the strain accounts for 30.28 percent, and the cucumber fusarium wilt can be prevented and controlled effectively.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Trichoderma strain highly effective against cucumber wilt disease and its application. Background technique [0002] Cucumber (Cucumis sativus L.) is one of the main melon vegetables grown in protected cultivation in my country. Due to the difficulty in reversed stubble and the increasingly serious obstacles to continuous cropping in protected cultivation, cucumber wilt, as a common and difficult to control soil-borne disease, seriously affects cucumber production. Cucumber Fusarium wilt, also known as vine wilt and wilting disease, is a soil-borne and root-invasive vascular disease. The general incidence is 20%-30%, and the severe plot is as high as 80%-90%, or even completely destroys the species. In recent years, due to the expansion of cucumber cultivation area in my country, cucumber wilt has occurred almost all over the country, among which 18 provinces...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCY02A50/30
Inventor 陈捷孙瑞艳刘志诚李雅乾姚力美林振亚
Owner SHANGHAI JIAO TONG UNIV
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