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Grape rhizosphere antagonistic growth-promoting Streptomyces F2 and application thereof

A grape root, streptomyces technology, applied in the application, bacteria, biocide and other directions, to achieve the effect of pepper growth promotion

Active Publication Date: 2021-12-14
DONGGUAN AGRI SCI RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening of antagonistic growth-promoting Streptomyces in grape rhizosphere soil has not been studied yet

Method used

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  • Grape rhizosphere antagonistic growth-promoting Streptomyces F2 and application thereof
  • Grape rhizosphere antagonistic growth-promoting Streptomyces F2 and application thereof
  • Grape rhizosphere antagonistic growth-promoting Streptomyces F2 and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Separation, purification and preservation of bacterial strains

[0033] Under sterile conditions, weigh 10g of grape rhizosphere soil sample and dissolve it in a 90mL Erlenmeyer flask filled with sterile glass beads and sterile water, shake the mixture on a vibrating screen at 180r / min for 3h, and gradually dilute into 10 -3 、10 -4 、10 -5 1 times the grape soil sample suspension, and then 100 μL of each dilution was evenly spread on the Gaoshi No. 1 medium plate, cultured at 28°C for 7 days, and the morphology was observed at any time. Pick the largest single bacterium colony on the culture medium, carry out four-section line purification, repeat 3 times, and observe the purity of the colony with a microscope at the same time, until a pure culture is obtained. Finally, the obtained pure culture strain was transferred into glycerol with a final concentration of 30% and sealed and stored in a -20°C refrigerator.

Embodiment 2

[0034] Embodiment 2: bacterial strain identification

[0035] Morphological observation: observe the shape and color of Streptomyces colonies growing on the surface of Gao’s No. staining reactions, etc. A well-growing strain F2 was screened and purified from grape rhizosphere soil samples using Gao's No. 1 medium. F2 was cultured on Gao's No. 1 medium for 7 days, such as figure 1 The color of -A colony gradually changes from white to gray, and the white single colony is raised, with a fluffy surface, rounded edges, concentric ring patterns, and hyphae; the results of microscopic examination are as follows:figure 1 -B, F2 are Gram-positive bacteria, the spore filaments are helical, and the spores are oval.

[0036] Determination of 16S rDNA gene sequence and phylogenetic analysis: After extracting bacterial genomic DNA, universal primers for bacterial 16S rDNA (27F: 5′-AGAGTTTGATCCTGGCTCAG-3′, 1492R: 5′-TACGGCTACCTTGTTACGACTT-3′) were used. After the amplification was comple...

Embodiment 3

[0041] Embodiment 3: Streptomyces antagonism to plant fungal disease

[0042] Streptomyces fermentation broth and sterile filtrate preparation: Streptomyces was inoculated in actinomycetes liquid culture medium, 28 ℃, 130r / min shake culture 24h as seed liquid, the seed liquid was transferred by the inoculation amount of 1.5% (volume ratio) Connected to actinomycetes liquid culture medium, 28 ℃, 130r / min shaking culture 5d, the fermentation liquid of the bacterial strain is obtained. Subsequently, the fermentation broth was centrifuged at 4° C. at 9000 r / min for 20 min, and the supernatant was filtered with a 0.22 μm sterile filter membrane to obtain a sterile filtrate of the bacterial strain.

[0043] Determination of the antibacterial spectrum of the fermentation broth: According to the method of Rong Liangyan et al., the antagonism between Streptomyces and pathogenic fungi was determined by the plate confrontation method. Inoculate the fungus and Streptomyces on the PDA pla...

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Abstract

The invention discloses grape rhizosphere antagonistic growth-promoting Streptomyces F2 and application thereof. The Streptomyces F2 is Streptomyces cajeri, has a preservation number of GDMCC No: 61143 and is preserved in Guangdong Microbial Culture Collection Center on August 14, 2020, the address is Floor 5, Building 59, #100 Courtyard, Middle Xianlie Road, Guangzhou City, and the Streptomyces F2 is classified and named as Streptomyces sp. Fermentation liquor and sterile filtrate of the strain F2 have antagonistic effects on phytophthora capsici and other 10 phytopathogens, and the highest bacteriostasis rate reaches 80% or above, wherein the fermentation liquor and the sterile filtrate have excellent inhibitory effects on the phytophthora capsici, and the inhibition rates are 80.58% and 87.71% respectively. The strain F2 fermentation liquor has a control effect on phytophthora capsici mycelium blocks. The strain F2 fermentation liquor has control effect of 61.09% on phytophthora capsici sclertium. The strain F2 fermentation liquor has an obvious control effect on phytophthora capsici, and the highest control effect reaches 83.31%. The F2 has the effects of dissolving organic phosphorus and fixing nitrogen, and can secrete extracellular protease and produce IAA. The root irrigation treatment of the strain F2 fermentation liquor has a remarkable promotion effect on the growth of chilies. The Streptomyces F2 not only has a broad-spectrum antagonistic plant pathogenic bacterium effect, but also has a growth-promoting effect.

Description

technical field [0001] The invention relates to plant rhizosphere growth-promoting bacteria, in particular to a growth-promoting streptomyces and application thereof. Background technique [0002] Soil-borne diseases caused by soil pathogens attacking crops from roots or stems are important types of agricultural plant pathogens, and the control of soil-borne diseases is an important limiting factor for the sustainable development of agriculture. The degree of damage of soil-borne diseases is related to the composition and concentration of the excretion of pathogenic bacteria in the soil. Therefore, inhibiting the activity of pathogens in the rhizosphere system becomes the basis for the prevention and control of soil-borne diseases. The restrictive effect of the relationship between plants, soil microorganisms and soil pathogens. Some scholars define the phenomenon and ability of the rhizosphere ecosystem to resist the invasion of soil pathogens as rhizosphere immunity. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P1/00A01P3/00A01P21/00A01G13/00A01G7/06C05F11/08C05G3/60C12R1/465
CPCA01N63/28A01G13/00A01G7/06C05F11/08C05G3/60
Inventor 胡珊梁卫驱黄皓徐匆罗华建胡楚维莫坚强黄晓彦陈仕丽
Owner DONGGUAN AGRI SCI RES CENT
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