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Application of citronellal in preparation of preparation for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa

A technology of Pseudomonas aeruginosa and citronellal, applied in the directions of applications, disinfectants, botanical equipment and methods, etc., can solve the problem of difficult eradication of Pseudomonas aeruginosa infection, and achieve inhibition of virulence and pathogenicity Effect

Inactive Publication Date: 2021-01-15
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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AI Technical Summary

Problems solved by technology

Pseudomonas aeruginosa infection is difficult to eradicate due to its inherent drug resistance and the tendency to form drug-resistant biofilms (Galloway et al., 2012)

Method used

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  • Application of citronellal in preparation of preparation for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa
  • Application of citronellal in preparation of preparation for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa
  • Application of citronellal in preparation of preparation for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa

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Embodiment 1

[0017]Preparation of PAO1 bacteria [Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1] suspension: sample the PAO1 culture solution in the exponential growth phase, centrifuge, wash once with PBS buffer, resuspend in PBS, and dilute the bacterial concentration to 108CFU / mL to obtain PAO1 bacterial suspension.

[0018]1. The effect of citronellal on the growth of Pseudomonas aeruginosa PAO1

[0019]Add LB medium and citronellal to the test tube respectively, and insert the PAO1 bacterial suspension in the exponential growth phase, the total volume is 10mL, so that the bacterial concentration of PAO1 is 106The concentrations of CFU / mL and citronellal were 0 (control), 0.27mg / mL, 0.54mg / mL, 1.08mg / mL and 2.15mg / mL. Take samples from several experimental groups and add them to a honeycomb culture plate dedicated to an automatic growth curve analyzer (Bioscreen C), add 350 μL of culture medium to each well, and three parallel for each experimental group. Place the honeycomb culture plate in...

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Abstract

The invention discloses an application of citronellal in preparation of a preparation for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa. What is found is that citronellal cannot inhibit growth of a pseudomonas aeruginosa PAO1 strain but can significantly inhibit las, rhl and pqs of three quorum sensing systems of the strain, and meanwhile generation of toxicity factors regulatedand controlled by the three quorum sensing systems is inhibited, and extracellular protease LasA, elastase LasB, pyocyanine, a biofilm, chitinase ChiC, exotoxin A and LecB and the like are included. Therefore, the strain can be used for inhibiting toxicity and pathogenicity of pseudomonas aeruginosa and reducing harm to infected hosts.

Description

Technical field:[0001]The invention belongs to the technical field of the prevention and control of harmful microorganisms, and specifically relates to the application of citronellal in preparing preparations for inhibiting the virulence and pathogenicity of Pseudomonas aeruginosa.Background technique:[0002]Pseudomonas aeruginosa is a common gram-negative bacterium, and it is also a common pathogenic bacterium for human conditions in clinic. Due to its inherent drug resistance and easy formation of drug-resistant biofilms, the infection of Pseudomonas aeruginosa is difficult to eradicate (Galloway et al., 2012). Therefore, the development of a new method to treat Pseudomonas aeruginosa infection is imminent. In recent years, quorum sensing (QS) has become a potential target for the treatment of pathogenic bacteria. Quorum sensing is defined as the intercellular communication between bacteria that uses small molecules that are easy to diffuse into the environment. When the number of ...

Claims

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Application Information

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IPC IPC(8): A01N35/02A01P1/00A61K31/11A61P31/04
CPCA01N35/02A61K31/11A61P31/04
Inventor 李文茹谢小保施庆珊曾桃花张志清刘静霞
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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