Novel preparation for inhibiting botrytis cinerea and application thereof

By combining isoeugenol and Bacillus belye to prepare a novel formulation with oil and aqueous phases, the problems of drug resistance and poor stability of traditional antibacterial agents are solved, achieving highly efficient antibacterial activity against Botrytis cinerea and effective control of gray mold, which is suitable for the planting and storage of high-sugar fruits.

CN122250461APending Publication Date: 2026-06-23CHINA THREE GORGES UNIV
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Patent Information

Application Number
CN202610298431.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-12
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing chemical pesticides are not very effective in inhibiting Botrytis cinerea (gray mold) and are prone to developing resistance. In addition, traditional plant essential oils have poor stability and are difficult to continuously and effectively inhibit the growth of Botrytis cinerea.

Method used

A novel formulation was prepared by combining isoeugenol and Bacillus belysinus to produce an oil-phase and an aqueous-phase product. Isoeugenol was used as the active ingredient in the oil phase, and the fermentation broth of Bacillus belysinus BV-J11 was used as the active ingredient in the aqueous phase. The synergistic effect of these components was achieved through emulsification technology. Rhamnol and gellan gum were used for encapsulation during the preparation process to enhance stability.

Benefits of technology

It achieves highly effective antibacterial activity against Botrytis cinerea, and isoeugenol masks the odor of Bacillus belye. The formulation significantly inhibits gray mold during the cultivation and storage of high-sugar fruits, and has good water solubility and dilution stability, providing an environmentally friendly antibacterial solution.

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Abstract

The invention discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of antibacterial drugs, and the key point of the technical scheme is as follows: the active ingredients of the preparation consist of isoeugenol and bacillus velezensis fermentation liquor. The half inhibitory concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the antibacterial ability of the novel preparation is higher than that of the existing bellkute in the prior art under the same concentration. Based on the influence on the membrane permeability of filamentous fungi, the invention can synergistically inhibit the pathogenic activity of the postharvest fungi of the fruits, and is expected to prepare a novel preparation for postharvest prevention and treatment of the high-sugar-degree fruits. The fruit preservative is easy to use and environmentally friendly, can be directly smeared on the surfaces of fruits in the planting stage, can not make direct contact with the fruits in the storage period, and provides the bacteriostatic ability for the storage period in a fumigation mode. A new choice is provided for biological control of fruits, and good economic benefits and social benefits are achieved.
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