Exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively

A technology of phthalic acid monoester and Lactobacillus plantarum, which is applied in the field of exopolysaccharide-producing Lactobacillus plantarum to achieve good adsorption capacity

Active Publication Date: 2017-12-29
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the food industry, there is no effective way to remove phthalate contamination in food
At present, there are no li...

Method used

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  • Exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively
  • Exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively
  • Exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively

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Experimental program
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Embodiment Construction

[0023] 1. Sample source

[0024] Functional lactic acid bacteria screening samples included Sichuan traditional family-made kimchi, fermented dairy products, fermented meat products and animal manure, etc. The strain in this case was derived from Sichuan traditional family-made kimchi. Sichuan kimchi is a traditional fermented vegetable product with Chinese characteristics, which is rich in lactic acid bacteria.

[0025] 2. Isolation and screening of lactic acid bacteria from different samples

[0026] Take different samples such as kimchi, fermented milk, animal manure, etc., and homogenize them in a sterile homogeneous bag at a ratio of 1:10. Take 1 mL of the homogenate and add 9 mL of sterile saline for 10-fold gradient dilution, and take the appropriate dilution 100 μL of sample was coated on MRS-CaCO 3 Medium plates, cultured at 37 °C for 24 h to 48 h. Pick colonies with calcium-dissolving circles and typical colony characteristics of lactic acid bacteria, perform mul...

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Abstract

The invention relates to the biotechnical field, particularly relates to the field of production and development of functional lactic acid bacteria, more particularly relates to exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively. The strain number of the exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively is RS20D, and the exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.13272 and the preservation date of November 14, 2016. The lactobacillus plantarum RS20D provided by the invention is functional lactic acid bacteria which has acid resistance, grows well in an environmental condition of pH 3.0-9.0, and particularly has the characteristic of adsorbing monophthalate and producing exopolysaccharides, for example, the lactobacillus plantarum RS20D is developed to a health-care food or drug which is taken for a long time to improve intestinal flora, and the damage of a phthalate plasticizer on a human body is reduced; or, the lactobacillus plantarum RS20D is input to a fermenting diary product or a cheese product, so that the sensory characteristic of the product can be improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of production and development of functional lactic acid bacteria. Specifically, it relates to a strain of exopolysaccharide-producing lactobacillus plantarum that effectively absorbs phthalic acid monoester. Background technique [0002] Lactic acid bacteria exopolysaccharide (LAB EPS) is a mucus or capsular polysaccharide secreted by lactic acid bacteria during growth and metabolism. The lactic acid bacteria strains producing exopolysaccharides can be directly applied to the production of fermented milk to replace or reduce the use of thickeners, improve the viscosity, texture and taste of yogurt products, and prevent shrinkage and whey precipitation. At the same time, the exopolysaccharide of lactic acid bacteria also has good physiological activities, such as anti-tumor, immune regulation and anti-oxidation, and can also be used as a prebiotic to promote the growth of o...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23C9/123A61K35/747A61P1/00C12R1/25
CPCA23C9/123A23L33/135A61K35/747A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/30A23V2200/3204
Inventor 刘书亮祝元婷周康敖晓琳何利陈姝娟杨勇邹立扣
Owner SICHUAN AGRI UNIV
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