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Bacillus cereus and application of bacillus cereus as agricultural biological agent

A technology of Bacillus cereus and biological agents, which is applied in the fields of chemicals, biocides, and microorganism-based methods for biological control, and can solve the problem of single function of microbial engineering strains

Active Publication Date: 2015-10-21
哈尔滨绿洲之星生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a strain of Bacillus cereus and its use as an agricultural biological agent in order to solve the problem that the current microbial engineering strains that degrade organophosphorus pesticides have a single function

Method used

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  • Bacillus cereus and application of bacillus cereus as agricultural biological agent
  • Bacillus cereus and application of bacillus cereus as agricultural biological agent
  • Bacillus cereus and application of bacillus cereus as agricultural biological agent

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Experimental program
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specific Embodiment approach 1

[0019] Specific Embodiment 1: In this embodiment, Bacillus cereus (Bacillus cereus) WSW-P05 is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No. 10605.

[0020] In this embodiment, Bacillus cereus (Bacillus cereus) WSW-P05 is isolated from the soybean rhizosphere soil in the Heihe Wudalianchi region of Heilongjiang by the microbial plate dilution method and the phosphorus-dissolving transparent delineation screening method.

[0021] In this embodiment, Bacillus cereus (Bacillus cereus) WSW-P05 is screened and obtained according to the following steps:

[0022] The soybean rhizosphere soil collected in Wudalianchi, Heihe River, Heilongjiang was air-dried, ground, and sieved, and 10.0 g of the sieved soil was placed in 100 mL of sterile water with glass beads to make a sample bacterial suspension, and placed on a constant temperature shaker at Fully shake at 180r / min for 30 minutes, and...

specific Embodiment approach 2

[0025] Specific embodiment two: In this embodiment, Bacillus cereus (Bacillus cereus) WSW-P05 is used as an agricultural biological agent.

Embodiment 1

[0027] Lecithin was used as the organophosphate source, and Ca 3 (PO 4 ) 2 As an inorganic phosphorus source, the phosphorus-solubilizing performance of the strain was measured (see the agricultural industry standard NY412-2000 for the measurement method).

[0028] Bacillus cereus (Bacillus cereus) WSW-P05 of the present invention is respectively inoculated in organic phosphorus liquid medium and inorganic phosphorus liquid medium with the inoculum amount of 0.5%, shakes under the condition that temperature is 30 ℃, rotating speed is 180r / min Cultivate for 72 hours; take 10 ml of the shaking culture solution and add 1 g of phosphorus-free activated carbon for decolorization, then centrifuge at 12000 r / min at low temperature for 20 min, take 5 ml of the supernatant, and use the molybdenum antimony scandium colorimetric method and Lambda 20 spectrophotometer at 680 nm to measure the content of available phosphorus. Determination.

[0029] The measurement result shows that the...

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Abstract

The invention provides bacillus cereus and an application of the bacillus cereus as an agricultural biological agent and relates to the bacillus cereus and the application of the bacillus cereus in the agricultural field. The bacillus cereus solves the problem that microorganism engineering strains for degrading organophosphorus pesticide are single in function in the prior art. The bacillus cereus WSW-P05 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacillus cereus WSW-P05 is CGMCC No: 10605. The bacillus cereus WSW-P05 serves as the agricultural biological agent. The bacillus cereus WSW-P05 serves as the agricultural biological agent so that crop growth can be promoted, and the crop yield can be increased; the organophosphorus pesticide in crops and soil is degraded; acid resistance is achieved; multiple pathogenic bacteria can be restrained; multiple functions are achieved.

Description

technical field [0001] The invention relates to a strain of bacillus cereus and its application in the agricultural field. Background technique [0002] The importance of pesticides in agricultural production is self-evident. According to statistics from the Food and Agriculture Organization of the United Nations, if pesticides are not used, the average annual loss caused by diseases, insects, and weeds in the world accounts for about 37% of crop production, and the annual loss is as high as 126 billion. Dollar. Organophosphorus pesticides, as a class of high-efficiency and broad-spectrum insecticides, are the most widely used pesticides in China. According to data from the China Pesticide Industry Association, the total output of pesticides in my country was 1.12 million tons in 2012, an increase of 11% over 2011, of which organophosphorus accounted for 70% of the total, and 12 varieties of raw materials produced more than 10,000 tons. 11 herbicides and 1 herbicide. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/085
CPCA01N63/00C12N1/205C12R2001/085
Inventor 吴皓琼牛彦波殷博安琦王笑庸曹亚彬郭丽姝樊川
Owner 哈尔滨绿洲之星生物科技有限公司
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