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Aminothiopropionic acid fast detecting method based on gold nano particle colloidal sols absorption spectrum

A gold nanoparticle and cysteine ​​technology, which is applied in the field of rapid detection of cysteine ​​based on gold nanoparticle sol absorption spectroscopy, can solve the problems of cumbersome operation process, complicated and expensive equipment and time-consuming, etc.

Inactive Publication Date: 2009-04-15
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to propose a rapid detection method of cysteine ​​based on the absorption spectrum of gold nanoparticle sol, to overcome the shortcomings of complex and expensive instruments and equipment, cumbersome operation process and long time-consuming in the existing detection technology

Method used

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  • Aminothiopropionic acid fast detecting method based on gold nano particle colloidal sols absorption spectrum
  • Aminothiopropionic acid fast detecting method based on gold nano particle colloidal sols absorption spectrum
  • Aminothiopropionic acid fast detecting method based on gold nano particle colloidal sols absorption spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: detect cysteine ​​concentration with the gold nanoparticle sol that pH value is 3.8

[0017] Step 1, preparation of gold nanoparticle sol:

[0018] The gold nanoparticle sol can be directly used as a commercial product, or can be obtained by self-made.

[0019] The specific steps of self-made gold nanoparticle sol are as follows: add 48mL deionized water and 2mL concentration of 25mM chloroauric acid solution in a 100mL round bottom flask, place it in a 90-95°C water bath to reflux for 5 minutes, then quickly add 5mL concentration of 38.8 mM trisodium citrate solution, and continued to reflux in the above-mentioned water bath for 20 minutes under vigorous stirring to obtain a deep red sol liquid. Remove the water bath, continue to stir, and cool to room temperature to obtain the gold nanoparticle sol.

[0020] Step 2, the preparation of buffer solution A with a pH value of 3.8:

[0021] The buffer solution with a pH value of 3.8 can be directly used as ...

Embodiment 2

[0030] Example 2: Detection of cysteine ​​concentration with gold nanoparticle sol with a pH value of 5.0

[0031] Step 1 is the same as Step 1 in Example 1.

[0032] Step 2, preparation of buffer solution D with a pH value of 5.0:

[0033] The buffer solution with a pH value of 5.0 can be directly used as a commercial product, or can be self-made. The specific steps for making a buffer solution with a pH value of 5.0 are as follows: Weigh 2.7343 g of sodium acetate crystals and dissolve them in 20 mL of deionized water, and add 954 μL of glacial acetic acid to the concentrated sodium acetate solution to obtain a mother liquor. Transfer the stock solution to a volumetric flask and dilute to 100 mL with deionized water to obtain 500 mM buffer solution D.

[0034] Step 3, preparation of cysteine ​​detection solution:

[0035] The specific operation steps are the same as Step 3 in Example 1, except that the buffer solution A in Example 1 needs to be replaced with buffer soluti...

Embodiment 3

[0041] Embodiment 3: detect cysteine ​​concentration with the gold nanoparticle sol that pH value is 5.7

[0042] Step 1 is the same as Step 1 in Example 1.

[0043] Step 2, the preparation of buffer solution F with a pH value of 5.7:

[0044] The buffer solution with a pH value of 5.7 can be directly used as a commercial product, or can be obtained by self-made. The specific steps for making a buffer solution with a pH value of 5.7 are as follows: Weigh 3.6914 g of sodium acetate crystals and dissolve them in 20 mL of deionized water, and add 286 μL of glacial acetic acid to the concentrated sodium acetate solution to obtain a mother liquor. Transfer the stock solution to a volumetric flask and dilute to 100 mL with deionized water to obtain 500 mM buffer solution F.

[0045] Step 3, preparation of cysteine ​​detection solution:

[0046] The specific operation steps are the same as Step 3 in Example 1, except that the buffer solution A in Example 1 needs to be replaced wit...

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Abstract

The invention discloses a cysteine quick detection method based on an absorption spectrum of Au nanoparticle collosol. The invention is characterized in that deionized water, buffer solution with 3.8 to 5.7 pH value and Au nanoparticle collosol stock solution are mixed to be prepared into detection solution, and the concentration of the buffer substance and the element Au in the detection solution is respectively 10 plus or minus 1mm and 0.9 plus or minus 0.1mm; the cysteine-containing liquid to be detected is added to the detection solution, the concentration of the cysteine in the formed detection system is 0.5 to 10mum, and the absorption spectrum is measured within ten minutes after even mixing; and according to the difference of the specific value eta of the absorbance of 700 nanometers and 525 nanometers of the wavelength in the absorption spectrum of the detection system, the concentration of the cysteine can be detected quantitatively. The method greatly reduces the time required for detecting the cysteine, and meanwhile, has the advantages of simple equipment, simple operation and mass detection.

Description

technical field [0001] The invention belongs to the technical field of cysteine ​​detection methods, in particular to a rapid detection method for cysteine ​​based on gold nanoparticle sol absorption spectrum. Background technique [0002] According to "Amino Acids" (edited by Wu Xianrong, Beijing Agricultural University Press, published in 1988, page 3), cysteine ​​is a sulfur-containing amino acid that plays an important role in the oxidation-reduction process of organism metabolism. According to "The Source of Human Biology—Amino Acids" (edited by Zou Zhongmei and Yu Changyuan, China Medical Science and Technology Press, published in 2000, pages 63-67), cysteine ​​and its derivatives are also potential neurotoxins and Biomarkers of various physiological environments, and their content in organisms are directly related to cardiovascular function. According to the United States "New England Journal of Medicine" (The New England Journal of Medicine 338:1042-1050, 1998), cys...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 曾杰吴呈昊刘彬于晓芳王晓平
Owner UNIV OF SCI & TECH OF CHINA
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