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Acinetobacter sp.Bap30 capable of effectively degrading benzo(a)pyrene and application thereof

A technology of benzo and bacilli, which is applied in the field of benzo[a]pyrene high-efficiency degradation strains, can solve the problems of low biodegradation efficiency of benzo[a]pyrene, and achieve the effect of improving the degradation rate

Inactive Publication Date: 2011-09-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a high-efficiency benzo[a]pyrene degrading bacterium and its application for the problem of low biodegradation efficiency of benzo[a]pyrene

Method used

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  • Acinetobacter sp.Bap30 capable of effectively degrading benzo(a)pyrene and application thereof
  • Acinetobacter sp.Bap30 capable of effectively degrading benzo(a)pyrene and application thereof
  • Acinetobacter sp.Bap30 capable of effectively degrading benzo(a)pyrene and application thereof

Examples

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Effect test

Embodiment 1

[0057] Example 1: The separation and purification method of bacterial strain Acinetobacter sp.Bap30 and the performance of degrading pyrene

[0058] 1. Separation and purification method for efficiently degrading benzo[a]pyrene strain Bap30

[0059] The method has the following steps:

[0060] 1. Dissolve 10 mg of benzo[a]pyrene in 50 mL of acetone solution so that the final concentration of benzo[a]pyrene is 200 mg / L;

[0061] 2. Take an appropriate amount of absorbent cotton, soak it in the acetone solution of the above-mentioned benzo[a]pyrene, and place it overnight;

[0062] 3. Wrap the absorbent cotton with a net bag the next day, tie the net bag with a strong long rope, and throw it into the sewer of the family area of ​​the East Campus of China Agricultural University;

[0063] 4. After standing for 21 days, take out the absorbent cotton and place it in a 250mL Erlenmeyer flask with 50mL acclimation medium. The active ingredient of the acclimatization medium is (NH ...

Embodiment 2

[0072] Example 2: Effects of different initial pH values ​​on the growth and degradation of benzo[a]pyrene of bacterial strain Acinetobacter sp.Bap30

[0073] Regulate inorganic salt medium (the composition of this medium is: (NH 4 ) 2 SO 4 1g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, FeCl 3 0.5g, CaCl 2 0.5g, benzo[a]pyrene 0.04g, distilled water 1000mL), the pH values ​​are 5.5, 6.0, 7.0, 8.0, 9.0, 9.5 respectively, the initial concentration of benzo[a]pyrene is 40mg / L, and the inoculum size is 5% (V / V), 160r / min, shake culture at 37°C for 7 days, measure the OD value of bacteria and the residual rate of benzo[a]pyrene, the results are shown in figure 2 .

[0074] Depend on figure 2 It can be seen that when the pH is 6.0, the degrading bacteria have the strongest degradation effect on benzo[a]pyrene, and the degradation rate is 17.89% after 7 days. When the pH is 9.5, the degradation rate is greatly inhibited, only 1.57% , indicating that the strain is ...

Embodiment 3

[0075] Embodiment 3: the influence of different inoculum sizes on the growth of bacterial strain and degradation benzo[a]pyrene

[0076] In the inorganic salt culture solution (the composition of this culture medium is: (NH 4 ) 2 SO 4 1g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.1g, FeCl 3 0.5g, CaCl 2 0.5g, benzo[a]pyrene 0.04g, distilled water 1000mL, pH value 7.0), the residual rate of benzo[a]pyrene after 7 days was 98.03%, 93.27%, 87.46%, 85.09% and 89.21% respectively ( See image 3 ). Depend on image 3 It can be seen that within a certain range, the larger the inoculum size, the higher the bacterial density, and the higher the degradation rate of the strain, but the larger the inoculum size, the better. low rate.

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Abstract

The invention discloses a bacterium for degrading benzo(a)pyrene and application thereof. The bacterium is acinetobacter sp.Bap30, CGMCC No.4586. The strain can grow with benzo(a)pyrene as the unique carbon source and energy, undergoes shake culture at the temperature of 37 DEG C for 20 days in the inorganic salt culture medium with benzo(a)pyrene concentration being 40mg / L and has degradation rate toward benzo(a)pyrene being 28.66%. The degradation rate of the strain toward benzo(a)pyrene can be effectively improved by adding defined amount of co-metabolic carbon sources, such as cane sugar and maltose. When another polycyclic aromatic hydrocarbon phenanthrene is taken as a co-metabolic substrate and exists by being mixed with benzo(a)pyrene, the degradation rate of the strain toward benzo(a)pyrene can be improved to 48.87% and meanwhile, the strain can realize complete removal of phenanthrene. The strain provided by the invention can provide new microorganism resources for degradation of polycyclic aromatic hydrocarbons in the water environment or soil environment.

Description

technical field [0001] The invention relates to the field of environmental refractory organic matter treatment, in particular to a benzo[a]pyrene highly efficient degrading strain and application thereof. Background technique [0002] Benzo[a]pyrene is an organic compound with obvious teratogenicity, carcinogenicity and mutagenicity. It is a polycyclic aromatic hydrocarbon compound composed of a benzene ring and a pyrene molecule. Benzo[a]pyrene is mainly produced from the incomplete combustion of hydrocarbons such as petroleum, coal, wood, gas fuel and paper and their thermal decomposition during the reduction process; therefore, benzo[a]pyrene is widely present in coal Tar, all kinds of carbon black, coal, petroleum, etc., in the smoke, cigarette smoke, and automobile exhaust, as well as industrial sewage such as coking, oil refining, asphalt, and plastics. Benzo[a]pyrene in surface water mainly comes from, besides industrial sewage, rainwater washing the atmosphere, leac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/20C02F101/32
Inventor 倪晋仁朱婷婷
Owner PEKING UNIV
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