Streptomyces parvus strain and application thereof

A technology of Streptomyces minor and pathogenic bacteria, applied in the field of microorganisms, can solve the problems of scarcity of agricultural antibiotics

Inactive Publication Date: 2011-09-14
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Herbimycin (Herbimycin) is an antibiotic found in the screening of herbicides, and it is effective against tobacco mosaic virus (TMV). Antibiotics; most of the antibiotics with antiviral activity were discovered during the screening process of anticancer substances; Japan and the United States are advanced representatives of agricultural antibiotic research and development, a

Method used

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  • Streptomyces parvus strain and application thereof
  • Streptomyces parvus strain and application thereof
  • Streptomyces parvus strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1, isolation, screening and identification of bacterial strains

[0024] 1. Isolation of strains

[0025] A strain Yn168 was isolated by pure culture from grassy slope soil of Dongbagu Mountain in Lijiang, Yunnan Province. Bacterial strain Yn168 was inoculated on Gaoshi No. 1 slant medium (Gaoshi No. 1 slant medium: starch 2.0g, agar 1.2g, KNO 3 0.1g, NaCl 0.05g, K 2 HPO 4 0.05g, MgSO 4 ·7H 2 O 0.05g, FeSO 4 ·7H 2 O 0.001g, water 100ml, pH 7.4~7.6, sterilized at 121°C for 30min. ) in a 28°C incubator for 15 days in the dark. Store at 4°C after removal.

[0026] 2. Strain identification

[0027] The morphological characteristics, culture characteristics, physiological and biochemical characteristics, and 16SrDNA sequence analysis and identification of strain Yn168 are as follows:

[0028] 1) Morphological characteristics of the bacteria

[0029] Bacterial strain Yn168 was respectively inoculated on GYM agar (recipe: glucose 4.0g, yeast extract 4.0g...

Embodiment 2

[0048] Example 2, Yn168 fermentation extract antiviral activity detection

[0049] 1. Preparation of Yn168 fermentation extract

[0050] 1) Obtaining the seed solution

[0051] Inoculate Streptomyces parvus Yn168 CGMCC No.4570 on the seed medium (seed medium: glucose 2.0g, NaCl 1.0g, peptone 0.6g, yeast powder 0.1g, water 100ml, natural pH, 121°C, 30min Sterilization), at 28°C, 200r / min conditions, constant temperature shaking culture for 24h, to obtain seed liquid.

[0052] 2) fermentation

[0053] The seed liquid that above-mentioned 1) obtains is inserted in the fermented medium by 5% inoculum size (fermented medium: 4.0g soluble starch, 4.0g glucose, 3.0g soya bean flour, 1.5g peptone, 0.5g yeast powder, 0.2g NaCl, 0.5g CaCO 3 , supplemented with water to 100ml, pH natural, 121°C, 30min sterilized), at 28°C, 200r / min (radius of rotation: 13mm) and constant temperature shaking culture for 6 days, centrifuge the fermentation product obtained above at 5000rpm / min for 15mi...

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Abstract

The invention discloses a Streptomyces parvus strain and application thereof. The collection number of the Streptomyces parvus Yn168 is CGMCC No.4570. Through the experiment, the Streptomyces parvus Yn168 strain (CGMCC No.4570), which is derived from soil and has antiviral activity, is separated, has important instructive significance for screening and developing antiviral active substances from microorganism resources, and also lays an experimental basis for separation and purification research of antiviral active substances of the Streptomyces parvus Yn168.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Streptomyces minor and its application. Background technique [0002] Actinomycetes, as the first discovered microorganisms with biocontrol effects, are also the most potential biological resources for the development of antiviral active substances among microorganisms. With the progress of actinomycetes biodiversity research, it provides a great possibility for the discovery of new biologically active substances. Antibiotics and non-antibiotics produced by many actinomycetes have a certain degree of control effect on plant viruses. [0003] Streptomyces is an important microbial group that produces the most antibiotics. Studies have shown that antibiotics are mainly produced by actinomycetes, and 90% of them are produced by Streptomyces. Currently, about two-thirds of clinically used antibiotics are derived from Streptomyces. genus; as a microbial group with...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/465
Inventor 刘伟成田兆丰刘建华裘季燕刘霆卢彩鸽刘德文
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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