Array-type high-throughput microbe separating culturing apparatus

A technology for separating and culturing and culturing devices, applied in enzymology/microbiology devices, biochemical cleaning devices, methods of supporting/immobilizing microorganisms, etc.

Active Publication Date: 2014-06-18
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These defects make the above-mentioned device not finally form a commercial product

Method used

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  • Array-type high-throughput microbe separating culturing apparatus
  • Array-type high-throughput microbe separating culturing apparatus
  • Array-type high-throughput microbe separating culturing apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, a kind of matrix type diffusion type microbial cultivation device

[0096] see figure 1, provide a kind of matrix type separation culture device including environment simulation device 1 and the culture device 2 (cultivation room 2) that is arranged in the environment simulation device 1, culture room 2 provides microorganism growth space; Environment simulation device 1 is used for supplying microorganism growth related environmental components. When in use, the culture chamber 2 is placed in the environment simulation device 1, and a complete set of devices is formed after the cover is covered.

[0097] see figure 2 , the culture chamber 2 is a "sandwich" structure, consisting of two layers of fixed plates, two layers of sealing plates, one layer of culture plates and two layers of filter membranes; from top to bottom are the upper fixed plate 21 and the upper sealed plate 22 , Upper filter membrane 23, intermediate culture plate 24, lower filter mem...

Embodiment 2

[0103] Embodiment 2, the combination of each part of matrix type diffusion type microbial cultivation device

[0104] see figure 1 , the culture device 2 and the environment simulation device 1 are fixed together, the nutrients can be exchanged between the two chambers through the filter medium (upper and lower filter membranes), the microorganisms are limited in the holes of the diffusion chamber, and the number of microorganisms in each hole can be determined according to the research Human control is required. The medium in the well can be selected in different forms or compositions (such as semi-solid or liquid) according to the research purpose, and the filling of the medium can match a multi-channel pipette or an automated pipetting workstation.

[0105] The environment simulation device 1 may contain seawater, fresh water, soil, etc. from the sampling environment, or may be a solution artificially prepared according to these environmental characteristics. The environme...

Embodiment 3

[0107] Embodiment 3, the mode of operation of matrix type diffusion type microbial cultivation device

[0108] see Figure 5 , the working process of the device of the present invention is that the cultivation chamber 2 is placed on the bracket 12 in the liquid storage tank 11, and a cavity is formed between the bottom of the cultivation chamber 2 and the inner bottom of the liquid storage tank 11, and the liquid storage tank 11 It is filled with liquid or semi-solid solution, that is, environmental water or mixture 15, which comes from seawater, river water, lake water in the natural environment of the microorganism to be separated; or a mixture of water and mud; or artificially prepared culture fluid, etc. Because each diffusion chamber on the intermediate culture plate 24 separates microorganisms (or other cells) from the external environment by two layers of filter membranes close to it, the cells are limited in the diffusion chambers 210, 211, and the diffusion chamber 21...

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Abstract

The invention relates to an array-type high-throughput microbe separating culturing apparatus, and discloses a novel microbe separating culturing apparatus. The apparatus is capable of realizing high-throughput separation and culture of cultured and / or uncultured microbes in a laboratory, operation is simplified, and the apparatus has good compatibility with conventional laboratory equipment. The separating culturing apparatus is capable of simulating in-situ culturing of microbe by natural environment, and is capable of efficiently separating difficult-to cultured microbe ( or named uncultured microbe or unculturable microbe). The microbe separating culturing apparatus is applicable to separate and culture difficult-to cultured microbe from environment, also is applicable to research interacting relationship between microbe and microbe, between microbe and environment factors, and between microbe and nutrition factors, and also is applicable to fields such as microbe source investigation and research, environment and ecology relationship, natural medicine discovery, biological technology research and the like.

Description

technical field [0001] The invention belongs to the field of microorganism cultivation; more specifically, the invention relates to an array type microorganism separation and cultivation device. Background technique [0002] There are abundant microbial species resources in the natural environment, which is the key to the discovery of drugs, the mining of functional microorganisms (such as bioenergy, biochemical industry, and biomaterial production bacteria), and to solve the major sustainable development issues facing human society today, such as resources, environment, population, and health. A treasure trove of resources for questions. It is known that the types of microorganisms that humans can cultivate are only 0.01-0.1% of marine microorganisms, and about 1% of terrestrial microorganisms. Many microbial taxonomy (Phylum) or class (Class) have not yet isolated a representative strain (Rappé , M.S, et al. The uncultured microbial majority. Annual Review of Microbiology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/12
CPCC12M23/12C12M23/34C12M29/04
Inventor 王勇郝玉有
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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