Application of Alternaria alternata metabolic product in controlling watermelon fusarium oxysporum
A technology of watermelon Fusarium wilt and Alternaria, which is applied in the field of microorganisms and can solve the problems of unsafe microbial source pesticide types and other issues
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Embodiment 1
[0018] Embodiment 1: (isolation and screening of Alternaria 31)
[0019] The present invention collected camphor tree stems in Tianmu Mountain, Hangzhou, Zhejiang in June 2008, and obtained Alternaria sp. 31. Save.
[0020] Determination of the antibacterial activity of Alternaria 31: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or watermelon wilt fungus (Fusarium oxysporium) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4mm respectively, and the fungus cake is connected to the center of the petri dish (petri dish 9 cm in diameter), placed in an incubator for 72 hours at 28°C, treated with sterile water as a control, and repeated 3 times; the diameter of the colony was measured by the cross method, and the inhibition rate was calculated by the foll...
Embodiment 2
[0023] Embodiment 2: (the preparation method of Alternaria 31 fermentation metabolite)
[0024] Follow these steps:
[0025] (1) Activation culture of strains: move the isolated and preserved Alternaria 31 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C ± 1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;
[0026] (2) Fermentation culture: The fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH per 1000mL. 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 126 hours;
[0027] (3) Extraction of fermented product: After the fermentation is ...
Embodiment 3
[0029] Embodiment 3: (the inhibitory effect of Alternaria 31 metabolites to cucumber solanum blight, watermelon wilt and tomato Botrytis cinerea)
[0030] (1) Accurately weigh 0.18 g of the metabolite of Alternaria 31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother solution with a concentration of 18 g / L;
[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1800mg / L and 900mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C (Final concentrations of Alternaria 31 metabolites are 180mg / L and 90mg / L), and after cooling, place a 4mm-diameter watermelon wilt or cucumber blight or tomato Botrytis cinerea on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator...
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