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Application of Alternaria alternata metabolic product in controlling watermelon fusarium oxysporum

A technology of watermelon Fusarium wilt and Alternaria, which is applied in the field of microorganisms and can solve the problems of unsafe microbial source pesticide types and other issues

Inactive Publication Date: 2011-10-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of endophytic fungus-Alternaria alternata (Alternaria alternata) that isolates and screens out from camphor tree for chemical pesticide unsafe and microbial source pesticide kind less; Another purpose of the present invention is Provides a method for preventing and treating plant fungal diseases by utilizing the metabolites of the strain

Method used

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  • Application of Alternaria alternata metabolic product in controlling watermelon fusarium oxysporum
  • Application of Alternaria alternata metabolic product in controlling watermelon fusarium oxysporum
  • Application of Alternaria alternata metabolic product in controlling watermelon fusarium oxysporum

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Embodiment 1

[0018] Embodiment 1: (isolation and screening of Alternaria 31)

[0019] The present invention collected camphor tree stems in Tianmu Mountain, Hangzhou, Zhejiang in June 2008, and obtained Alternaria sp. 31. Save.

[0020] Determination of the antibacterial activity of Alternaria 31: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or watermelon wilt fungus (Fusarium oxysporium) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4mm respectively, and the fungus cake is connected to the center of the petri dish (petri dish 9 cm in diameter), placed in an incubator for 72 hours at 28°C, treated with sterile water as a control, and repeated 3 times; the diameter of the colony was measured by the cross method, and the inhibition rate was calculated by the foll...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Alternaria 31 fermentation metabolite)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Alternaria 31 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C ± 1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;

[0026] (2) Fermentation culture: The fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH per 1000mL. 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 126 hours;

[0027] (3) Extraction of fermented product: After the fermentation is ...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Alternaria 31 metabolites to cucumber solanum blight, watermelon wilt and tomato Botrytis cinerea)

[0030] (1) Accurately weigh 0.18 g of the metabolite of Alternaria 31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother solution with a concentration of 18 g / L;

[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1800mg / L and 900mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C (Final concentrations of Alternaria 31 metabolites are 180mg / L and 90mg / L), and after cooling, place a 4mm-diameter watermelon wilt or cucumber blight or tomato Botrytis cinerea on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator...

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Abstract

The invention relates to the technical field of microorganisms. The camphor tree endophytic fungi disclosed by the invention is separated from a camphor tree plant living body collected in the Tianmu Mountain in Hangzhou city of Zhejiang province through use of an endophytic fungus microorganism separation technology and is called after Alternaria alternata 31 through microbial taxonomic identification; and the strain is preserved in the General Microorganism Centre of China Microorganism Strain Preservation Management Committee on December the 31st, 2010 and has a preservation registered number of CGMCC No.4508. The strain liquid is obviously characterized by generating an active metabolic product which has the inhibiting effect on plant fungus disease pathogens, such as cucumber rhizoctonia solani, watermelon fusarium oxysporium and tomato botrytis cinerea, and the like through fermenting and is an important microbial resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of the biocontrol endophytic fungus Alternaria alternata and the application of its metabolites. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12P1/02C12R1/645
Inventor 俞晓平申屠旭萍董胜张郝培应边亚琳马正
Owner CHINA JILIANG UNIV
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