Biological process oil extraction method for prolonging period of validity by use of microbe to drive oil
A microbial oil recovery and microbial flooding technology, which is applied in the direction of production fluid, earth drilling, wellbore/well components, etc., can solve problems such as imbalance of nutrient composition, waste of microbial resources, and influence on the ability and effect of microorganisms
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[0013] The present invention is described further below:
[0014] First, microorganisms and nutrient solution are injected from the microbial injection well, and then water is injected; the production fluid of the beneficial oil production well is periodically tested, and the sampling period of the production fluid is 7-15 days. The samples taken are placed in clean glassware and analyzed within 24 hours. Microbial concentration analysis was carried out on the production fluid samples of benefit oil production wells by the plate viable culture counting method; the glycolipid surfactants in the production fluid samples of benefit oil production wells were analyzed by the "sulfuric acid-phenol" method; the "acidification precipitation" was adopted The method was used to analyze the lipopeptide surfactant in the output fluid sample of the benefit oil production well; the acid-base titration method was used to analyze the total amount of organic acids in the output fluid sample of...
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