Salt-tolerant aerobic denitrifying bacterium and application

An aerobic denitrifying bacteria and salt-tolerant technology, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as the impact of denitrification treatment process, and achieve good growth performance

Active Publication Date: 2019-03-19
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the presence of salt, the nitrogen rem

Method used

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  • Salt-tolerant aerobic denitrifying bacterium and application

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Embodiment 1

[0011] Example 1: Screening of salt-tolerant aerobic denitrifying bacteria.

[0012] For realizing above-mentioned object, the technical scheme that the present invention takes is:

[0013] (1) Enrichment and acclimatization: 10 mL of tannery wastewater was added to 90 mL of sterilized enrichment medium, and after 48 h of shaker culture (30 o C, 150 rpm), transfer 10 mL of cell suspension to a new 90 mL sterilized enrichment medium, and carry out secondary enrichment culture under the above culture conditions. Transfer 10 mL of the secondary enrichment medium to a new 90 mL sterilized enrichment medium, and complete the third enrichment acclimatization under the same conditions

[0014] (2) Primary screening: The third enrichment culture solution was serially diluted, and plated onto solid selection medium with 2% agar (30 o C, 48 h), according to the discoloration of the selection medium, select the blue single colony that appears on the medium, streak the single colony on ...

specific Embodiment 2

[0028] Specific Example 2: Growth and denitrification performance of salt-tolerant aerobic denitrifying bacteria under different salt concentration conditions.

[0029] Growth of salt-tolerant aerobic denitrifying bacteria in different concentrations of NaCl and degradation of nitrate nitrogen. The DN-23 seed liquid was inoculated into the denitrification medium containing different salt concentrations (0%, 3%, 4%, 5%, 6%) according to the inoculum amount of 1%, at 30 o C, cultivate 48 h in the shaker of 150 rpm, according to [0016] method, use ultraviolet spectrophotometer to detect biomass (OD 600 ) and the remaining nitrate nitrogen mass concentration in the medium, and the trend of the two with the NaCl concentration is obtained, such as figure 1 shown. With the increase of salt concentration, the biomass decreased, but the degradation rate of nitrate nitrogen was still at a high level. At the same time, it still had good growth performance under the condition of 6% NaC...

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Abstract

The invention discloses a salt-tolerant aerobic denitrifying bacterium and an application thereof. The bacterium can be used for efficiently removing nitric nitrogen in high-salt wastewater, is namedas Pseudomonas sp. DN-23 and collected in a China Center for Type Culture Collection on May 21, 2018, and the collection number of the bacterium is CCTCC NO: M2018290. Acclimating, preliminary screening and secondary screening are performed to obtain the salt-tolerant aerobic denitrifying bacterium, and a new microorganism resource is provided. The screened Pseudomonas is acquired from leather wastewater and can grow well in a high-salt environment, and the nitric nitrogen in a water body can be effectively removed. The bacterium is applied to the treatment process of the high-salt wastewater,and has a wide prospect.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology and the field of environmental protection. It specifically relates to a salt-tolerant aerobic denitrification bacterium and its application in denitrification of high-saline water. Background technique [0002] With the rapid development of industry and agriculture, the discharge of nitrogen-containing organic matter into water bodies in the process of production and living has increased rapidly, and the discharge of a large amount of nitrogen-containing organic matter has caused serious water eutrophication and water pollution. How to efficiently remove nitrogen from water has become a hot research topic. For a long time, the traditional denitrification theory believed that the enzyme system of denitrifying bacteria was in O 2 It will be inhibited when it exists, and only under anaerobic conditions can denitrifiers use NO 3 - , NO 2 - Acts as an electron acceptor for denitrificati...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
CPCC02F3/34C02F2101/163C12N1/205C12R2001/38
Inventor 吴重德李丹粱锡宏李政威
Owner SICHUAN UNIV
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