Bacillus tequilensis S12 for preventing and treating rice blast and application thereof

A rice blast and bacillus technology, which is applied in the directions of application, bacteria, fungicides, etc., to achieve the effects of easy acquisition of culture conditions, inhibition of pathogenicity, and good prospects for development and application

Pending Publication Date: 2020-04-10
长春沃谷科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although plant protection workers have isolated a number of biocontrol strains that can significantly inhibit the growth and development of rice blast fungus, there is still a long way to go to effectively pr

Method used

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  • Bacillus tequilensis S12 for preventing and treating rice blast and application thereof
  • Bacillus tequilensis S12 for preventing and treating rice blast and application thereof
  • Bacillus tequilensis S12 for preventing and treating rice blast and application thereof

Examples

Experimental program
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Embodiment 1

[0044] This embodiment provides a preparation method of boiled potato filtrate: take 200 g of potatoes, wash, peel, and cut into pieces, add 800 g of water to boil for 20 minutes, and obtain the boiled potato filtrate after filtering;

[0045] Also provided is a PDA culture solution with a pH value of 7.0: the components include 20% potato boiled filtrate, 2% glucose, 2% agar and 78% purified water;

[0046] Also provided is a PDB culture solution with a pH value of 7.0: the components include 20% potato boiled filtrate, 2% glucose and 78% purified water.

[0047] The present embodiment also provides a preparation method of a biocontrol agent for effectively preventing and treating rice blast, and the specific steps include:

[0048] (1) Activate the Bacillus tequila S12 strain on a PDA plate medium at 28°C for 24h, pick a single colony and cultivate it on a PDA slant medium at 25°C for 18h to obtain the cultured strain;

[0049] (2) inoculate the cultured strain into the PDB...

Embodiment 2

[0052] This embodiment provides a preparation method of boiled potato filtrate: take 200 g of potatoes, wash, peel, and cut into pieces, add 1000 g of water to boil for 30 minutes, and obtain the boiled potato filtrate after filtering;

[0053] Also provided is a PDA culture solution with a pH value of 7.2: the components include 20% potato boiled filtrate, 2% glucose, 2% agar and 78% purified water;

[0054] Also provided is a PDB culture solution with a pH value of 7.2: the components include 20% potato boiled filtrate, 2% glucose and 78% purified water.

[0055] The present embodiment also provides a preparation method of a biocontrol agent for effectively preventing and treating rice blast, and the specific steps include:

[0056] (1) Activate the Bacillus tequila S12 strain on a PDA plate medium at 34°C for 20h, pick a single colony and cultivate it on a PDA slant medium at 35°C for 12h to obtain the cultured strain;

[0057] (2) Inoculate the cultured strain into the PD...

Embodiment 3

[0060] This embodiment provides a preparation method of boiled potato filtrate: take 200 g of potatoes, wash, peel, and cut into pieces, add 900 g of water to cook for 25 minutes, and obtain the boiled potato filtrate after filtering;

[0061] Also provided is a PDA culture solution with a pH value of 7.1: the components include 20% potato boiled filtrate, 2% glucose, 2% agar and 78% purified water;

[0062] Also provided is a PDB culture solution with a pH value of 7.1: the components include 20% potato boiled filtrate, 2% glucose and 78% purified water.

[0063] The present embodiment also provides a preparation method of a biocontrol agent for effectively preventing and treating rice blast, and the specific steps include:

[0064] (1) Activate the Bacillus tequila S12 strain on a PDA plate medium at 31° C. for 22 hours, pick a single colony and cultivate it on a PDA slant medium at 30° C. for 15 hours to obtain the cultured strain;

[0065] (2) Inoculate the cultured strai...

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Abstract

The invention relates to bacillus tequilensis S12 for preventing and treating rice blast and an application thereof, and specifically discloses a bacillus tequilensis strain S12, and an application ofthe S12 strain in prevention and treatment of the rice blast. The strain is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 18347. Thebacillus tequilensis S12 strain and metabolites thereof provided by the invention have strong antagonistic effect on magnaporthe grisea, can significantly inhibit the pathogenicity of the magnaporthegrisea, thereby effectively preventing and treating the rice blast; thus, the bacillus tequilensis S12 strain and the metabolites thereof can be used for biological prevention and treatment of the rice blast. In addition, the biological agent, namely the bacillus tequilensis S12 provided by the invention is safe to human and livestock, is free of problems of environmental pollution and the like,has simple preparation method and low cost, and is simple to use.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to bacillus tequila S12 for preventing and treating rice blast and its application. Background technique [0002] Rice is one of the three cereal crops with an annual output of more than 50 million tons in the world, and it is the main source of basic food for more than 3 billion people in the world (especially in Asia) every day. Rice blast disease (rice blast disease) is one of the most serious rice diseases, which occurs in all rice producing areas in the world, and can cause a 10-30% reduction in rice production every year. All tissues and parts of rice can be affected. For example, leaf blast occurs on leaves, which is the most common disease, which can lead to death of leaves in severe cases. In addition to leaves, stems can also suffer from disease, forming node blast. Lodging and withering; when the disease occurs at the heading stage, it is ea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 史洋李柱刚张森林魏炜
Owner 长春沃谷科技有限公司
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