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Sanguinarine stimulates rice resistance and its application in rice blast resistance

A technology for resistance to rice blast and sanguinarine, applied in the application, chemicals for biological control, fungicides, etc., can solve the problem of unclear mechanism of action, and achieve the ability to improve the ability, inhibit the pathogenic process, and improve the expression. Effect

Active Publication Date: 2021-09-07
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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Problems solved by technology

Recent studies on Magnaporthe grisea have shown that sanguinarine can inhibit spore germination, promote the production of reactive oxygen species, change the morphology of the nucleus and the membrane potential of mitochondria, but its mechanism of action in the infection of Magnaporthe grisea and the defense of rice Not clear, there is no report on the application of sanguinarine in rice blast resistance

Method used

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  • Sanguinarine stimulates rice resistance and its application in rice blast resistance
  • Sanguinarine stimulates rice resistance and its application in rice blast resistance
  • Sanguinarine stimulates rice resistance and its application in rice blast resistance

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Embodiment

[0021] In the present invention, a certain concentration of sanguinarine is added to the culture medium in vitro, and the effect of sanguinarine on the mycelia growth of the pathogen on the culture medium is observed, and at the same time, a certain concentration of sanguinarine is added to the wild-type strain of Magnaporthe grisea, Guy11 In the conidia suspension of rice blast fungus, observe the appressorium formation of the pathogen on the hydrophobic glass slide, the pathogenic process and the expression of rice resistance response genes, and then determine the rice blast fungus conidia suspension containing sanguinarine. Plague pathogenicity.

[0022] Specifically include the following steps:

[0023] The effect of exogenous addition of sanguinarine on the mycelia growth of Magnaporthe grisea: Dissolve sanguinarine in methanol to a concentration of 3.8 mg / mL in the mother solution, add it to the basic medium, and prepare the final concentrations of 2.0 μM, 4.0 μM and 8....

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Abstract

The invention provides a compound sanguinarine and its anti-rice blast function. The compound exists in the rhizome of Papaveraceae Sanguinarum, and is a natural alkaloid with antibacterial, anti-inflammatory and anti-oxidative properties. Inhibit tumor cell proliferation and promote apoptosis. The present invention detects its effects on the mycelial growth, spore germination, appressorium development and pathogenicity of Magnaporthe grisea by adding sanguinarine compounds in vitro, and the results show that adding a certain concentration of sanguinarine from exogenous sources can effectively activate rice disease resistance-related genes expression, thereby significantly inhibiting the virulence of Magnaporthe grisea. The invention proposes the inhibitory effect of sanguinarine on the pathogenicity of rice blast fungus, indicating that the compound can be used for preventing and treating rice blast.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of exogenously adding a compound sanguinarine in rice blast resistance. Background technique [0002] Crop diseases caused by pathogenic fungi are one of the serious threats to global food production and food security. Fungal diseases are the largest category of plant diseases, accounting for about 70-80% of plant diseases. Many plant diseases with wide distribution and serious damage are caused by fungi. Rice is the most important food crop in my country. Nearly 60% of the country's population takes rice as a staple food. It can be seen that the production safety of rice is related to the national economy and people's livelihood of our country. Magnaporthe grisea ( Magnaporthe oryzae ) caused by rice blast is one of the devastating diseases in global rice production. It can occur in the whole development period of rice. %~30% yield loss, which seriously...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 威利汤蔚郑华坤陈美莲郭嘉媛陈奕晓朱尔斯石茗月张冬梅王宗华
Owner FUJIAN AGRI & FORESTRY UNIV
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