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Molecular marker of rice blast resistance gene pigm in Gumei 4 and its application
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A rice blast resistance gene and molecular marker technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. The effect of rice blast resistance
Active Publication Date: 2019-05-31
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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[0005] Purpose of the invention: In view of the lack of specificity of the markers in the prior art and the lack of close linkage with the target gene, one of the purposes of the present invention is to provide the molecular marker of Gumei No. 4 rice blast resistance gene Pigm, one of the purposes of the present invention The second is to provide primers for detecting the molecular markers, and the third object of the present invention is to provide the application of the molecular markers
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Embodiment 1
[0027] Example 1 Acquisition and verification of the specific genome sequence marker Pigm-4 of Gumei No. 4
[0029]The rice materials used in this study include the genomic DNA of the donor varieties of Pi9, Pi2, Piz, Pi-zt, Pi40: 75-1-127, C101A51, Toride 1, Fukunishiki, IR65482, and the donor Gumei No. 4 of Pigm ; 250 copies of recipient japonica rice line Wuyun 2674, indica recipient variety Zhenhui 82, conventional japonica rice varieties and typical hybrid rice maintainer and restorer lines (9311, Nipponbare, Zhonghua 11, Xiushui 519, Longjing 41, Lianjing 4, Lianjing 6, Lianjing 7, Lianjing 11, Lianjing 9, Lianjing 10, Xudao 3, Huajing 3, Huajing 4, Huajing 5, Huajing No. 6, Huajing No. 7, Huaidao No. 5, Huaidao No. 6, Huaidao No. 7, Huaidao No. 8, Huaidao No. 9, Huaidao No. 10, Huaidao No. 11, Huainuo No. 12, Huaidao 13, Huaiyoujing 2, Yandao 6, Yandao 8, Yandao 9, Yandao 11, Yanjing 9, Yanjing 10, Yanjing 11, Yangyujing 2 No. 1, Yangnongdao ...
Embodiment 2
[0059] Example 2 Using the genome-specific marker Pigm-4 and the upstream marker Pigm-2 to transduce the Pigm(t) gene to breed new rice varieties with broad-spectrum blast resistance
[0060]The test material and test method of this embodiment are substantially the same as in Example 1, the used donor variety is Gumei No. 4, and the recipient variety is Wuyun 2674 (one participated in the rice regional test in Jiangsu Province but was eliminated because of rice blast failure. The new strain of Wu 2674, also known as Wu 2674, can be obtained by those skilled in the art through conventional means. In addition, the recipient varieties are not limited to Wu 2674), Zhenhui 82, and the detection markers are the two wing markers Pigm-2 and Pigm-4, rice blast The method of inoculation identification and evaluation refers to the literature of Deng, Wu et al. . Theor Appl Genet, 2006, 113:705–713.
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Abstract
The invention discloses a molecular marker of rice blast resistant gene Pigm of Gumei 4 and an application thereof. The molecular marker comprises a specific molecular marker Pigm-4 of Gumei 4, wherein the molecular marker Pigm-4 comprises the DNA fragments different in the 10436119-10436192bp of the chromosome 6 of Nipponbare genome, while the sequence of the section of Gumei 4 is lost; and the forward and reverse primers of the Pigm-4 primer pair are shown by SEQ ID No:2 and SEQ ID No:3 respectively. The forward and reverse primers of the Pigm-2 of the forward molecular marker primer pair of Pigm gene are shown by SEQ ID No:4 and SEQ ID No:5 respectively. The invention also discloses an application of the molecular marker in improving the rice blast infection resistance of rice through a molecular marker assisted selection of rice blast resistant rice breeding and a molecular marker assisted selection method. The molecular marker disclosed by the invention has good specificity and realizes tighter interlocking with a target area.
Description
technical field [0001] The invention belongs to the technical field of rice molecular breeding, and more specifically, the invention relates to a molecular marker of the rice blast resistance gene Pigm of Gumei No. 4 and an application thereof. Background technique [0002] Rice blast is one of the three major diseases of rice production worldwide, and cultivating disease-resistant varieties is the most economical and effective means to control rice blast damage. Traditional rice disease resistance breeding is phenotypic selection of plants through resistance identification, which takes a long time and is easily limited by environmental conditions. The identification results are prone to errors and the selection efficiency is low. The molecular marker-assisted selection of disease-resistant genes is not affected by the above-mentioned unfavorable factors, and is one of the effective ways to breed disease-resistant varieties. [0003] Gumei 4 is a local variety with broad-sp...
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