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Rice Gene osgdsl1 and Its Application to Resistance to Blast

A rice blast resistance, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of improving the ability of rice blast resistance

Active Publication Date: 2020-07-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Currently, there is no information on rice OsGDSL1 Application of Genes in Rice Blast Resistance

Method used

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  • Rice Gene osgdsl1 and Its Application to Resistance to Blast
  • Rice Gene osgdsl1 and Its Application to Resistance to Blast
  • Rice Gene osgdsl1 and Its Application to Resistance to Blast

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Experimental program
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Effect test

Embodiment

[0023] The present invention is constructed by OsGDSL1 The overexpression vector pCXUN-HA-OsGDSL1 was transformed into mature rice embryos through Agrobacterium-mediated genetic transformation. The background variety of the transformed rice was wild-type rice Nipponbare. Screened from the offspring OsGDSL1 Overexpressed strains were inoculated with Magnaporthe grisea to determine the resistance of rice to disease.

[0024] It includes the following steps:

[0025] OsGDSL1 Gene acquisition and construction of the overexpression vector pCXUN-HA-OsGDSL1: obtained from the rice genome database OsGDSL1 Coding sequence to OsGDSL1 The full-length coding sequence was used as a template for primer design, and the forward primer OsGDSL1-HA-F: 5'-ATGAGGCTCTCGGTCTCCGTCG-3' and the reverse primer OsGDSL1-HA-R: 5'-TCAGTGGTGGCGGGTCTCGAG-3' were obtained, and the wild-type rice Nipponbare (NPB) cDNA was used as a template and amplified with KODplus high-fidelity enzyme OsGDSL1 , After cutting and ...

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Abstract

The invention provides a rice gene OsGDSL1 and application thereof in resisting rice blast. The open reading frame of the gene is 1119 bp and encodes a GDSL-like lipase composed of 372 amino acids. The invention obtains the OsGDSL1 overexpressing rice plants by constructing the rice overexpression vector pCXUN-HA-OsGDSL1 by means of agrobacterium-mediated rice mature embryo transformation technology, and the rice blast fungus inoculation experiment is carried out on a wild type and rice plants with overexpressed OsGDSL1. The results show that the overexpression of OsGDSL1 increases the resistance of rice plants to magnaporthe oryzae, indicating that the gene can be used to breed rice varieties resistant to rice blast.

Description

Technical field [0001] The invention belongs to the field of rice cultivation, and specifically relates to a rice OsGDSL1 Application of gene and its overexpression vector in rice resistance to rice blast. Background technique [0002] Rice is one of the important economic crops. More than half of the world's population feeds on rice as the staple food. It occupies an extremely important position in food production. Rice blast caused by Magnaporthe grisea is the main disease that affects high-quality and high-yield rice. The disease has the characteristics of strong outbreak, which can lead to the catastrophic consequences of rice yield reduction or even no harvest. Currently, with people discovering that the rice blast fungus can infect gramineous crops such as wheat, barley, and corn, the disease has attracted more attention from the scientific community. For a long time, many scientists are committed to finding effective methods to prevent and control rice blast to alleviate ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/20C12N15/82A01H5/00A01H6/46
CPCC12N9/20C12N15/8282
Inventor 鲁国东宋林林韩艺娟林娅红王宗华
Owner FUJIAN AGRI & FORESTRY UNIV
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