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Sanguinarine-excited rice resistance and application thereof in resisting rice blast

A rice blast, rice blast resistance technology, applied in application, chemical for biological control, animal repellent, etc. Improve the effect of expression

Active Publication Date: 2021-02-05
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recent studies on Magnaporthe grisea have shown that sanguinarine can inhibit spore germination, promote the production of reactive oxygen species, change the morphology of the nucleus and the membrane potential of mitochondria, but its mechanism of action in the infection of Magnaporthe grisea and the defense of rice Not clear, there is no report on the application of sanguinarine in rice blast resistance

Method used

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  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast
  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast
  • Sanguinarine-excited rice resistance and application thereof in resisting rice blast

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Embodiment

[0021] In the present invention, a certain concentration of sanguinarine is added to the culture medium in vitro, and the effect of sanguinarine on the mycelia growth of the pathogen on the culture medium is observed, and at the same time, a certain concentration of sanguinarine is added to the wild-type strain of Magnaporthe grisea, Guy11 In the conidia suspension of rice blast fungus, observe the appressorium formation of the pathogen on the hydrophobic glass slide, the pathogenic process and the expression of rice resistance response genes, and then determine the rice blast fungus conidia suspension containing sanguinarine. Plague pathogenicity.

[0022] Specifically include the following steps:

[0023] The effect of adding exogenous sanguinarine on the mycelia growth of Magnaporthe grisea: Dissolve sanguinarine with methanol to a concentration of 3.8 mg / mL in the mother solution, add it to the basic medium, and prepare the final concentration of 2.0 μM, 4.0 μM and 8.0 μM...

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Abstract

The invention provides a sanguinarine compound and application thereof in resisting rice blast, the sanguinarine compound exists in the rhizome of opium poppy plant sanguinarine, is a natural alkaloidwith antibacterial, anti-inflammatory and antioxidant properties, and also has the effects of inhibiting tumor cell proliferation and promoting apoptosis. The influence of the sanguinarine compound added in vitro on magnaporthe grisea hypha growth, spore germination, appressorium development and pathogenicity is detected, and the result shows that the expression of rice disease resistance relatedgenes can be effectively activated by adding a certain concentration of sanguinarine to an external source so that the pathogenicity of magnaporthe grisea is remarkably inhibited. The invention provides an inhibition effect of sanguinarine on magnaporthe oryzae pathopoiesis, which indicates that the compound can be used for preventing and treating the magnaporthe oryzae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of exogenously adding a compound sanguinarine in rice blast resistance. Background technique [0002] Crop diseases caused by pathogenic fungi are one of the serious threats to global food production and food safety. Fungal diseases are the largest category of plant diseases, accounting for about 70-80% of plant diseases. Many plant diseases with wide distribution and serious damage are caused by fungi. Rice is the most important food crop in my country. Nearly 60% of the country's population takes rice as a staple food. It can be seen that the production safety of rice is related to the national economy and people's livelihood of our country. Magnaporthe grisea ( Magnaporthe oryzae ) caused by rice blast is one of the devastating diseases in global rice production. It can occur in the whole development period of rice. %~30% yield loss, which seriously t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 威利汤蔚郑华坤陈美莲郭嘉媛陈奕晓朱尔斯石茗月张冬梅王宗华
Owner FUJIAN AGRI & FORESTRY UNIV
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