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Application of baicalin in resisting and preventing bacterial infection

A technology of bacterial infection and baicalin, applied in antibacterial drugs, medical preparations containing active ingredients, respiratory diseases, etc., can solve the problem of unknown mechanism of action, and achieve the effect of inhibiting pathogenicity

Inactive Publication Date: 2010-09-29
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Modern Chinese pharmacy research has found that the chemical components of various traditional Chinese medicines can be used as natural antibacterial drugs to achieve antibacterial and therapeutic effects, including flavonoids contained in various traditional Chinese medicines, but their mechanism of action is unknown

Method used

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  • Application of baicalin in resisting and preventing bacterial infection
  • Application of baicalin in resisting and preventing bacterial infection
  • Application of baicalin in resisting and preventing bacterial infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0033]Construction of bacterial screening system for anti-pathogenic substances. Plasmid pMS402 is the vector for the reporter gene lux-CDABE with a deleted promoter. Toxicity-related genes include lasR, rhlI, rhlR, lasI, exoT, exsD, exoS, oprH, phzA1, phzA2, pilG, fliC, mig4, xcpR, aprA, rnr. The promoter region of the gene was amplified by PCR and cloned into the front of the reporter plasmid pMS402 luciferase gene operon by restriction digestion. The constructed recombinant plasmids were named pKD-lasR, pKD-rhlI, pKD-rhlR, pKD-lasI, pKD-exoT, pKD-exsD, pKD-exoS, pKD-oprH, pKD-phzA1, pKD-phzA2, pKD-pilG, pKD-fliC, pKD-migA, pKD-xcpR, pKD-aprA, pKD-rnr. After digesting the recombinant plasmid with PacI, the large fragment contains the promoter sequence of the lux reporter gene and the toxicity-related gene, and the size is about 8kb. Point-specific recombinant restriction enzyme digestion. The purified lux-CDABE fragment with the promoter-reporter fusion was cloned into th...

Embodiment approach 2

[0035] Solid plate screening:

[0036] Coarse sieving of solids by pointing filter paper on double-layer culture medium: Baicalin was found to inhibit the expression of the quorum sensing system of Pseudomonas aeruginosa and the expression of multiple virulence factors. Its chemical formula is as follows: figure 1 shown. Under the same conditions, these samples have basically no effect on the growth of bacteria. Listed are the inhibitory effects of baicalin on the expression of QS-related genes lasR, rhlI, rhlR, and lasI, three-type secretion system genes exoT, exoS, and phenazine synthetic gene phzA1. Figure, see figure 2 .

Embodiment approach 3

[0038] Liquid re-screening by enzyme label detector method: analyze the results of coarse screening, use enzyme label detector to re-screen various Chinese medicine ingredients in 96-well plates, and find baicalin, which has the ability to inhibit Pseudomonas aeruginosa quorum sensing system and other The role of multiple virulence factors in luminescence.

[0039] see Figure 3A (a), when the concentration of baicalin containing baicalin is 250 μg / mL, the inhibitory effect on the expression of the quorum sensing Rh1 system autoinducer synthase gene rhllI reaches more than 3 times; see Figure 3B (a) 2-fold inhibition of lasR gene expression; see Figure 3C (a) and Figure 3D (a), less than 2-fold inhibition of rhlR, lasI gene expression; see Figure 3E (a), the inhibitory effect on phzA1 gene expression reaches more than 8 times; see Figure 3F (a), the inhibitory effect on exoS gene expression reached more than 5 times; see Figure 3G (a), the inhibitory effect on exoT ...

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Abstract

The invention relates to application of baicalin in resisting and preventing bacterial infection and provides application of baicalin which does not bring survival pressure to bacteria and has little probability of generating resistance to drugs in resisting and preventing bacterial infection.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to the application of baicalin in antibacterial infection and prevention of bacterial infection. Background technique [0002] The emergence of bacterial resistance limits people's ability to treat bacterial infectious diseases and poses a serious threat to human health. With the continuous emergence of multiple drug-resistant strains, the treatment of bacterial infectious diseases with traditional antibiotics is facing great challenges. The reason for the emergence of multidrug-resistant pathogenic bacteria is due to the blind abuse of antibiotics in recent years on the one hand, and the mechanism of action of traditional antibiotics on the other hand. Since traditional antibiotics are aimed at inhibiting the growth or survival of bacteria, this puts a lot of pressure on the survival of the bacteria themselves, which leads to the emergence of bacterial resistance. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/04A61P11/00A61P43/00
Inventor 段康民迈克·G·撒瑞特黄一农郭俏
Owner NORTHWEST UNIV(CN)
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