Preparation method and use of high antiviral active recombination chicken gamma-interferon crCHIFN-gamma

An interferon and virus technology, applied in the field of the preparation of broad-spectrum high-efficiency antiviral genetic engineering products, can solve the problems of low rChIFN-γ activity and no antiviral activity, etc.
CN1657616AInactive Publication Date: 2005-08-24YANGZHOU UNIV
0 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2005-08-24
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells. It can be used to suppress the pathogenic action of both MDV GA to CEF and AIV(H5NI) virus to cell, and the reproduction of NDV F48E8 on cells.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a preparation method of a broad-spectrum high-efficiency antiviral genetic engineering product. Background technique

[0002] Chicken γ-interferon (ChIFN-γ) is a cytokine produced by activated T cells and NK cells stimulated by mitogens or specific antigens, with a molecular weight of 17-20KD. IFN-γ has biological activities such as anti-virus, anti-tumor cell growth and differentiation, and plays an important role in immune regulation. The ChIFN-γ gene was first cloned by Digby and Lowenthal in 1995, and then domestic and foreign scholars successively expressed ChIFN-γ with Escherichia coli, COS cells, baculovirus, fowl pox virus, avian adenovirus and plant tobacco mosaic virus. Biological activity has been extensively studied. The laboratory also successfully cloned the ChIFN-γ gene, and expressed and applied it in Escherichia coli and COS cells. It was found that the prokaryotic expressed rChIFN-γ had no antiviral activity...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More