Rice blast prevention and cure method

A technology of rice blast and rice, applied in the field of biological control of rice pests and diseases, can solve the problems of reducing control efficiency, polluting the environment, and easy variation, and achieves the effect of biological control

Inactive Publication Date: 2018-01-19
安徽省中日农业环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex genetic background of the rice blast fungus, it is easy to mutate, and it is difficult for the disease-resistant varieties to keep up with the variation speed of the physiological races of the pathogenic bacteria, which often leads to the loss of resistance of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A control method for rice blast, comprising the following steps:

[0022] Step 1: Select healthy rice leaves, cut them into 5cm leaf segments, put them into a triangular flask with 75% ethanol, shake and wash twice, pour off the ethanol, add sterile water to soak for 30 minutes, and then make a gradient dilution to obtain a gradient dilution liquid;

[0023] Step 2: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;

[0024] Step 3: Draw the gradient dilution and spread it on the NA solid medium. After drying, culture it upside down in a constant temperature incubator at 30°C for 48 hours, pick a single colony, and draw a gradient line on the NA solid medium to obtain a single colony again. Bacterial colonies, the obtained single colonies are preserved in the slant of NA sol...

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PUM

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Abstract

The invention discloses a rice blast prevention and cure method, and relates to the technical field of biological prevention and cure of rice diseases and pests. A preparation method of the rice blastprevention and cure method comprises the steps of gradient dilution solution preparation, NA solid medium preparation, bacillus separation, PDA medium preparation, spore solution preparation and riceplanting. According to the rice blast prevention and cure method, bacilli obtained through separation can secret antibacterial material to inhibit growth of pathogenic bacteria and induce plant defense systems to defend against invasion of the pathogenic bacteria; tested bacterial strains have significant inhibition action on germination of conidia of magnaporthe grisea, germ tube extending and appressorium forming; active substances of the bacterial strains have strong inhibition action on the magnaporthe grisea, and the inhibition rate of growth of magnaporthe grisea hyphae reaches 80% or above.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a method for preventing and treating rice blast. Background technique [0002] Rice is China's largest grain crop, and its output accounts for about 40% of China's grain output. The three major diseases of rice include rice blast, bacterial blight and rice sheath blight. Among them, rice blast is one of the most threatening diseases in rice production. It is caused by the fungus Pyrospora fungus and can occur throughout the rice development period. Due to the rapid prevalence of the disease, under suitable environmental conditions, it can cause a pandemic in a short period of time and bring huge losses to rice production. At present, in agriculture, disease-resistant breeding and chemical agents are mainly used to gradually control rice blast. However, due to the complex genetic background of Magnaporthe grisea, it is easy to mutate,...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01G13/00A01N63/02A01P3/00
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司
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