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Biocontrol bacillus subtilis BJ-1 and application thereof

A technology of Bacillus subtilis and fungus, which is applied in the field of agricultural microorganisms to achieve the effects of fast reproduction, high temperature storage and storage resistance

Active Publication Date: 2017-12-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of Bacillus subtilis fermentation broth for seed treatment to control rice blast

Method used

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  • Biocontrol bacillus subtilis BJ-1 and application thereof
  • Biocontrol bacillus subtilis BJ-1 and application thereof
  • Biocontrol bacillus subtilis BJ-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation, screening and fermentation of Bacillus subtilis BJ-1

[0027] 1. Isolation, identification and determination of antibacterial spectrum

[0028] The microorganism of the present invention is a Bacillus subtilis strain with strong antagonistic activity isolated from a naturally polluted tomato and oat plate in the Hubei Provincial Key Laboratory of Crop Disease Detection and Safety Control, Huazhong Agricultural University, Wuhan City, Hubei Province. Separation and purification refer to the plant disease research method (Fang Zhongda, 1998). The method is to pick a Bacillus subtilis colony from a naturally polluted plate, dilute it with 5ml sterile water, dip a small amount of diluent with an inoculation needle and draw it on a PDA plate. Line, select the desired characteristic single colony, after 3 times of the same streak culture, finally get the pure Bacillus subtilis strain. The applicant named it BJ-1, and finally identified the biocontrol ba...

Embodiment 2

[0039] Embodiment 2: Bacillus subtilis BJ-1 fermentation filtrate is to the inhibitory effect and teratogenic effect of blast fungus mycelia growth

[0040] 1. Inhibitory effect of Bacillus subtilis BJ-1 fermentation filtrate on the growth of blast fungus mycelia

[0041] After adding the Bacillus subtilis fermentation broth filtrate to PDB and mixing it into different concentrations (v / v, 0, 0.5%, 1%, 2.5%, 5%, 10%, 20%), the rice blast with a diameter of 5mm was beaten Mycelia blocks were inoculated in 75mL of PDB culture medium containing different concentrations of fermentation broth filtrates, treated without adding fermentation filtrates as a control, placed at 28°C and 180rpm for shaking culture for 7 days, and then used pre-dried and weighed qualitative filter paper (diameter 10cm) filter the mycelium, and place it in a 60°C drying oven to dry to constant weight, and weigh the dry weight of the mycelium with an electronic balance with an accuracy of one ten-thousandth ...

Embodiment 3

[0049] Example 3: Inhibition of Bacillus subtilis BJ-1 fermentation filtrate on blast fungus spore germination and appressorium formation

[0050] The Bacillus subtilis BJ-1 fermentation broth filtrate was diluted with 0.025% Tween to different concentrations (v / v, 1%, 2%, 5%, 10%, 20%, 40%) and then mixed with Tween 20 (final concentration 0.025%) at a concentration of 1×10 6 The equal volumes of the blast fungus spore liquid / ml are mixed, so that the concentration of the fermented filtrate contained in the spore liquid is respectively (v / v, 0.5%, 1%, 2.5%, 5%, 10%, 20%). Plague has a spore concentration of 5×10 5 spores / ml. The above-mentioned blast fungus spore liquid containing different concentrations of Bacillus subtilis fermentation broth filtrate was inoculated onto hydrophobic slides respectively, 4 drops per slide, 10 μl per drop. After inoculation, place them under constant temperature and dark conditions at 28°C for 18 hours of moisturizing culture, and then dir...

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Abstract

The invention discloses a biocontrol bacillus subtilis BJ-1 and an application thereof. The bacillus subtilis is preserved in China Center for Type Culture Collection (CCTCC) with preservation number of CCTCC NO: M2017443. The bacillus subtilis provided by the invention, as a biocontrol bacterium, has a good inhibitory effect on various fungi. With the application of the bacillus subtilis BJ-1 provided by the invention, mycelial growth, spore germination and appressorium formation of rice blast can be effectively inhibited; as rice seeds are treated by being soaked in a solution prepared from the bacillus subtilis, rice growth can be promoted and plant resistance can be induced, and potential adverse influence of chemical control on environment and rice safety and infection risk in using of a disease-resistant variety can be prevented; and in addition, the bacillus subtilis, which is rapid in reproduction, quite strong in stability, high-temperature resistant and storage resistant and free from toxic and side effects, can be processed and developed in the dosage forms of viable fungicides and metabolites.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a strain of Bacillus subtilis BJ-1 and its application in the prevention and treatment of rice blast. Background technique [0002] Rice is one of the most important food crops in the world, and nearly half of the world's population uses rice as the main food. In my country, the planting area of ​​rice accounts for about 1 / 4 of the country's cultivated land area, and the annual output is about 1 / 2 of the country's total grain output. Rice diseases are important limiting factors affecting rice yield and quality, and rice blast is one of the most widespread and harmful worldwide fungal diseases. Rice blast can cause a large reduction in rice production, and in severe cases, the production can be reduced by 40% to 50%, or even no grains can be harvested. It occurs in every rice area in the world, and most of them occur in leaves and nodes. After the...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 郑露何有为黄俊斌
Owner HUAZHONG AGRI UNIV
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