Preparation method of microbial pesticide for controlling rice blast
A technology for microbial pesticides and rice blast, which is applied in the field of biological control of rice diseases and insect pests, can solve the problems of reduced control effect, complex genetic background, reduced rice quality, etc., and achieves the effect of good biocontrol effect.
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[0021] A preparation method of a microbial pesticide for controlling rice blast, comprising the following steps:
[0022] Step 1: Select healthy rice leaves, cut them into 5cm leaf segments, put them into a triangular flask with 75% ethanol, shake and wash twice, pour off the ethanol, add sterile water to soak for 30 minutes, and then make a gradient dilution to obtain a gradient dilution liquid;
[0023] Step 2: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;
[0024] Step 3: Draw the gradient dilution and spread it on the NA solid medium. After drying, culture it upside down in a constant temperature incubator at 30°C for 48 hours, pick a single colony, and draw a gradient line on the NA solid medium to obtain a single colony again. Bacterial colonies, the obtained single colo...
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