Cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, extract, preparation method and application thereof

A technology of endophytic fungi and Chinese fir, which is applied in the field of fermentation filtrate and extracts of Chinese fir endophytic fungi, and can solve the problems of lack of research

Active Publication Date: 2020-10-23
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Epicoccum dendrobii It is an endophytic fungus of fir, but there is still a lack of research on the purification and extraction of antibacterial components of this fungus and the application of its extracts

Method used

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  • Cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, extract, preparation method and application thereof
  • Cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, extract, preparation method and application thereof
  • Cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, extract, preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: Chinese fir endophyte Epicoccum dendrobii SMEL isolation and identification

[0027] In this experiment, healthy Chinese fir branches were collected in Nanjing Forestry University in April 2017 (collector: Huang Lin;

[0028] Contact number: 025-85427301), wash with sterile water, and then sterilize the surface with 75% absolute ethanol, remove the epidermis, transfer the internal tissue to a PDA plate, culture at 28°C, and use sterile picks after 7 days. The edge part of the colony was picked with a needle and inoculated on a new PDA plate to purify the strain to obtain the endophytic fungus strain SMEL of Chinese fir.

[0029] endophytic fungi Epicoccum dendrobii The biological characteristics of the strain SMEL are as follows: The edges of the colony of the SMEL strain on the PDA medium are neat, and the aerial mycelium is felt to flocculent, flat, white to light brown; the back of the colony is light red, and the center is light brown to brown. T...

Embodiment 2

[0031] Embodiment 2: SMEL fermentation filtrate is to the inhibitory action of Chinese fir anthracnose spore germination and appressorium formation

[0032] Preparation of mycelial blocks: Inoculate C. fir anthracnose and SMEL on PDA plates respectively, culture them at 25°C for 5 days, and use a sterile puncher to prepare bacterial blocks with a diameter of 5 mm (ie, mycelial blocks).

[0033] In order to prepare the spores of the fungus Anthracnose fir (provided by the Forest Pathology Laboratory of Nanjing Forestry University, the strain name is SMCG1#C, by 2017. Observation of the nuclear behavior of the genetic transformation of the fungus anthracnose and appressorium development. Nanjing Forestry University Nanjing Forestry University Journal (Natural Science Edition), 41(6):68-72. Open) mycelium block was inoculated into 100ml CMC liquid medium, 25°C, 200rpm shaking culture, spores were collected after 2 days and the concentration was adjusted to 1×10 5 spores / ml, and ...

Embodiment 3

[0035] Embodiment 3: the inhibitory effect of the ethyl acetate extract of SMEL fermentation filtrate to the growth of Chinese fir anthracnose

[0036] Inoculate 10 pieces of SMEL mycelium with a diameter of 5mm into a 500ml Erlenmeyer flask containing 200ml of sterile PD medium, place in a darkroom shaker at 25°C, shake at 130rpm for 40d, and collect the fermentation filtrate by filtering through a funnel; The fermentation filtrate is filtered through a 0.22-μm filter to obtain the aseptic fermentation filtrate of SMEL (after filtration, the fermentation filtrate does not contain SMEL bacteria, and the components metabolized into the fermentation liquid during the SMEL cultivation process play an antibacterial role); to the sterile fermentation filtrate Add 3 times the volume of ethyl acetate and mix thoroughly (that is, the volume of ethyl acetate is 3 times the volume of the fermentation filtrate), and then let it stand for 24 hours; use a separatory funnel to collect the up...

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Abstract

The invention discloses a cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, an extract, a preparation method and an application thereof. The fermentation filtrate of the Epicoccum dendrobii SMEL disclosed by the invention can be used for remarkably inhibiting the colletotrichum gloeosporioides spore germination of the cunninghamia lanceolata and inhibiting the appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; the ethyl acetate extract of the Epicoccum dendrobii SMEL fermentation filtrate can obviously inhibit the mycelial growth ofthe colletotrichum gloeosporioides of the cunninghamia lanceolata, and obviously inhibit the spore germination and appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; and an inoculation test shows that the ethyl acetate extract obviously inhibits the pathogenicity of colletotrichum gloeosporioides of the cunninghamia lanceolata.

Description

technical field [0001] The invention belongs to the field of microbial pesticides, and in particular relates to a fermentation filtrate of fir endophytic fungi and its extract, preparation method and application. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) is widely planted in Fujian, Jiangxi, Guangxi, Sichuan, Hunan, Anhui, Jiangsu and other provinces (regions) in China. Due to its straight and round stem shape, fast growth speed, insect resistance and corrosion resistance, and high economic value, it is my country's Important fast-growing timber species. The afforestation area and timber volume of Chinese fir rank first among the main afforestation tree species in my country. Glosospora anthracnose ( Colletotrichum gloeosporioides ) caused by Chinese fir anthracnose has caused serious harm to the growth of Chinese fir in my country's fir cultivation areas. When the disease is mild, the needles or young shoots of Chinese fir seedlings will turn b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A01N63/30A01P3/00C12N1/14C12R1/645
CPCA01N63/30C12P1/02C12N1/145C12R2001/645
Inventor 黄麟卞金月方玉兰宋青孙美玲巨云为
Owner NANJING FORESTRY UNIV
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