Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A material for inducing appresses of corn leaf spot fungus and its preparation method and application

A technology of corn leaf spot fungus and resin, applied in the field of research on the infection mechanism of plant pathogenic fungi, can solve the problems of inability to build a high-throughput system, high brittleness of cellophane, cumbersome operation, etc., and achieve high induction efficiency, fast drying speed, and preparation simple effect

Active Publication Date: 2022-06-24
河北福赛农业科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the high brittleness of cellophane, it is easy to tear, and the operation of using it to induce appressoria is cumbersome. It cannot be covered on a 96-well plate for induction of appressoria of maize leaf spot, and a high-throughput system cannot be constructed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A material for inducing appresses of corn leaf spot fungus and its preparation method and application
  • A material for inducing appresses of corn leaf spot fungus and its preparation method and application
  • A material for inducing appresses of corn leaf spot fungus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A material for inducing appressorium of Pseudomonas maize, which is made from the following raw materials by volume:

[0048] 10mL of Si-PUD resin copolymerized with organosilicon diol and aliphatic isocyanate,

[0049] Self-curing liquid polytetrafluoroethylene resin 10mL.

[0050] The above-mentioned preparation method of the material for inducing the appressorium of Pseudomonas maize, comprises the following steps:

[0051] (1) Take 10 mL of Si-PUD resin copolymerized with silicone diol and aliphatic isocyanate, and 10 mL of self-curing liquid polytetrafluoroethylene resin, put them in a 50 mL beaker, and put a magnetic stirrer rotor into the beaker, The beaker was placed on a magnetic stirrer and stirred for 10 min to obtain a mixed coating;

[0052] (2) Use a pipette with a range of 5mL to draw 3mL of the mixed paint and evenly apply it to a petri dish with a diameter of 90mm, place the petri dish in a blast drying oven, dry at 80°C for 30min, and the paint is co...

Embodiment 2

[0056] The epiphyte induction experiment was carried out with B. maize as the test pathogen:

[0057] The conidia suspension of B. maize is added dropwise to the Si-PUD+PTFE material coating prepared in Example 1, and after induction at a constant temperature of 25°C for 12 hours, the induction is observed under an optical microscope;

[0058] And set up a control group that uses cellophane as a substrate for appressorium induction of B. maize:

[0059]Pour a small amount of water agar with a concentration of 2% into a petri dish with a diameter of 90mm, cover the bottom of the petri dish, and let it stand for 20 minutes. After the water agar solidifies, spread the cellophane on the water agar, and drop the same amount of corn on the cellophane The conidium suspension of B. spp. was kept at a constant temperature of 25°C in the dark for 12 hours, and the induction was observed under a light microscope.

[0060] like figure 1 As shown, the conidia of B. maize was cultured on ...

Embodiment 3

[0062] The epiphyte induction experiment was carried out with Pseudomonas maize as the test pathogen:

[0063] The suspension of conidia of P. zeaeii was added dropwise to the Si-PUD+PTFE material coating prepared in Example 1, and after induction at a constant temperature of 25°C for 12 hours, the induction was observed under an optical microscope;

[0064] And set up a control group that uses cellophane as a substrate for appressorium induction of B. zeae:

[0065] Pour a small amount of water agar with a concentration of 2% into a petri dish with a diameter of 90mm, cover the bottom of the petri dish, and let it stand for 20 minutes. After the water agar solidifies, spread the cellophane on the water agar, and drop the same amount of corn on the cellophane Microspot conidia suspension was kept at a constant temperature of 25°C in the dark for 12 hours, and the induction was observed under a light microscope.

[0066] Set up the control group of Si-PUD resin copolymerized w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a material for inducing appresses of corn leaf spot fungus and its preparation method and application, and belongs to the technical field of research on the infection mechanism of plant pathogenic fungi. The material is made of the following raw materials by volume: 4-6 parts of Si-PUD resin copolymerized with silicone diol and aliphatic isocyanate, and 4-6 parts of self-curing liquid polytetrafluoroethylene resin. The materials for inducing appresses of corn leaf spot fungus of the present invention have safe raw materials, simple preparation, and convenient use, are suitable for high-throughput systems, are used for inducing appresses of corn leaf spot fungi, have high induction efficiency, and are suitable for the infection mechanism of corn leaf spot fungus. Research.

Description

technical field [0001] The invention belongs to the technical field of plant pathogenic fungal infection mechanism research, and in particular relates to a material for inducing appressorium of corn leaf spot fungus and a preparation method and application thereof. Background technique [0002] Corn leaf spot is an important disease that harms corn production. The main pathogenic fungi that cause corn leaf spot are B. maize and B. maize. The key to maize leaf spot infection of maize leaf spot lies in the appressorium. The huge turgor pressure in the appressorium makes the maize leaf spot fungus form an invading nail to penetrate the host epidermis, thereby infecting the leaf. Therefore, the development of materials for inducing appressorium formation of P. maize is beneficial to better study the infection mechanism of P. maize. [0003] The commonly used material in the laboratory to induce the appressorium formation of P. maize is cellophane; when using cellophane to induc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C09D175/04C09D127/18
CPCC09D175/04C09D127/18C08L27/18C08L75/04
Inventor 曾凡力肖生林董金皋孟亚南李盼郝志敏
Owner 河北福赛农业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com