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SNP molecular markers related to chicken-fertilization duration time characters and application thereof

A technology of duration and molecular markers, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., which can solve the problems of troublesome measurement and large economic investment.

Active Publication Date: 2016-04-20
湖北欣华生态畜禽开发有限公司
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AI Technical Summary

Problems solved by technology

However, this trait is cumbersome to measure, requiring single-cage rearing, individual tracking, and large economic investment; there are few reports on this trait from a genetic perspective

Method used

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  • SNP molecular markers related to chicken-fertilization duration time characters and application thereof
  • SNP molecular markers related to chicken-fertilization duration time characters and application thereof
  • SNP molecular markers related to chicken-fertilization duration time characters and application thereof

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Embodiment Construction

[0036] The invention provides a SNP molecular marker related to chicken fertilization duration traits, the chicken fertilization duration traits related SNP molecular marker is chicken fertilization duration in the first intron of chicken TGFB3 (transforming growth factor-β3) gene Trait-associated SNP molecular markers.

[0037] The nucleotide sequence of the SNP molecular marker related to chicken fertilization duration traits provided by the present invention is SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4;

[0038] When the SNP molecular marker associated with chicken fertilization duration traits is SEQ ID NO: 1, the nucleotide sequence of SEQ ID NO: 1 is:

[0039] TCCTCTGCCTCAGGCCAGAACAGCTTGGCTCCCTAGTGCCGGCCCCAGCRTTCGGTGCAAGGCCACAGGTGAGAGGGAGGAGATCCCATACTTTGCTCCA;

[0040] The 50th base R in the sequence of SEQ ID NO: 1 is C or T, resulting in polymorphism;

[0041] When the SNP molecular marker associated with chicken fertilization duration traits is SEQ ID...

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Abstract

The invention provides SNP molecular markers related to chicken-fertilization duration time characters and an application thereof. TGFB3 genes are subjected to polymorphic researching and association analysis with the association analysis method, and the SNP molecular markers related to the chicken-fertilization duration time characters are found from first introns of the chicken TGFB3 genes for the first time; the SNP molecular markers particularly relate to four SNP molecular markers related to hen-fertilization duration time characters and the positioning and the application of a haplotype formed by the four SNP molecular markers. The SNP molecular markers are obtained through association analysis, and the nucleotide sequences of the SNP molecular markers are shown as SEQ ID NO:1-4. The molecular markers and the haplotype can be used for molecular breeding of chicken-fertilization duration time.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and specifically relates to four SNP molecular markers related to chicken fertilization duration traits, haplotypes formed by the four SNPs and applications thereof. Background technique [0002] Differences in the duration of fertilization traits (the number of days that fertilized eggs can be continuously produced after one artificial insemination) of hens directly affect the frequency of artificial insemination and the utilization rate of male hens. Thereby affecting economic benefits, it is an important reproductive trait in chicken production. In breeding chicken production, the frequency of artificial insemination is once every 5 days, and every 50 hens need to be equipped with a breeding rooster. If the trait is selected, the frequency of artificial insemination can be reduced, and the labor cost of artificial insemination can be reduced. It can also greatl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李世军辜澜涛孙成浩许东伟陈鹏杨刘斌龚炎长
Owner 湖北欣华生态畜禽开发有限公司
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