Separation method of xylem parenchyma cell protoplast of rice root
A technology of parenchyma cells and protoplasts, applied in plant cells and other directions, can solve problems such as few reports
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[0016] 1. Cultivation of the medium column protoplast isolation material:
[0017] Take one Petri dish of each size, spread two layers of filter paper in the small Petri dish, moisten it, spread a layer of rice seeds evenly and at appropriate intervals, cover the large Petri dish, and place it for 24 hours without light.
[0018] After the seeds germinated, they were sown in a cultivation pot filled with quartz sand, and the cultivation conditions were 255°C, 60% humidity, and 10 hours of sunlight. After a week, take out the seedlings and wash off the quartz sand.
[0019] 2. Separation of middle column:
[0020] Clean the root system of rice seedlings, take the thicker root system, remove the 1cm length of the tip and the part with root hairs on the upper part, and use the remaining 2-3cm as the source of the central column material.
[0021] Put the cut root in 0.2% CaCl2 solution, tap it gently with small tweezers, so that the root system is flat, use the tip of the tweez...
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