The invention belongs to the biological technical field, and in particular relates to screening of high-yield
glycine moulds and an application thereof in
nitrile compound conversion.
Glycine is widely applied to the fields such as food,
chemical industry,
medicine and the like; and the
glycine is produced by utilizing a
bioconversion method instead of a synthesis method, which becomes a development trend at home and abroad. The invention provides a
screening method of high-yield
glycine strains. By utilizing the method, a high-yield mould strain H3 for the glycine is screened for the first time, and the mould strain is identified as
Fusarium oxysporum by combining morphological, physiological and biochemical characters and sequencing of genes, namely 18S, ITS and 28S rDNA; and the strain is conserved at China General
Microbiological Culture Collection Center (conservation number: CGMCC 3829). The
Fusarium oxysporum H3 screened by the method has the characteristics of fast
growth speed, high conversion efficiency, short
production cycle, mild
reaction conditions, high yield, low production cost, environmental friendliness and the like, thus being applicable to industrial production of the glycine; and the after-treatment process of the product is convenient and fast without a large amount of acid or alkali.