Method for producing taxol by using taxus lateral root proliferation

Abstract

The invention discloses a method of utilizing taxus lateral root proliferation to produce paclitaxel, the steps are that: (1) annual or biennial taxus branches are selected as cutting materials; (2) after the cuttings are sequentially injected into KMnO4 solution, sucrose solution, and plant growth hormone solution for soaking, the cuttings are conveyed to cutting substrate; (3) the cuttings are pulled out after the roots grow up, and are continually conveyed to the cutting substrate after being processed with hormone; (4) a certain quantity of the lateral roots are cut for producing the paclitaxel; (5) being dried under temperature control at the temperature that is less than or equal to 55 DEG C, pure products of the paclitaxel are obtained after processes of grinding, concentrated organic solvent extraction, column chromatography, solvent recovery, crystallizing and drying; or the cut taxus lateral roots are cultured in a improved Murashige and amp;Skoog fluid nutrient medium, bacteriostat is added in the nutrient medium, 2 to 9 days after fresh taxus lateral roots are induced and processed by elicitors, the pure products of the paclitaxel are extracted. The invention provides a new approach for medicine sources of the paclitaxel, and the shortcomings in artificial cultivation of the taxus are solved of low rooting rate, low survival rate after the roots are cut, slow roots proliferation speed, etc.

Description

technical field

[0001] The invention belongs to the fields of agricultural biotechnology, biopharmaceuticals, forest product chemistry and industry, and specifically relates to a method for producing paclitaxel by multiplying lateral roots of yew. Background technique

[0002] Paclitaxel is currently an important anti-tumor drug recognized internationally, and it is also the only natural compound that can promote tubulin polymerization and inhibit its depolymerization. Due to its unique anti-tumor mechanism and significant anti-tumor activity, paclitaxel ranks first in the market share of anti-tumor drugs.

[0003] Currently, paclitaxel used in research and clinical trials is mostly extracted from the bark of natural yew plants. The paclitaxel content in the bark is very small, only 0.007-0.01% of the dry weight of the bark, and usually 15-30 tons of bark will be used for every 1 kg of pure paclitaxel extracted. According to calculations, six yew trees with an age of 60-10...