Method for producing taxol by using taxus lateral root proliferation

A taxus and paclitaxel technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of slow root proliferation, low survival rate, low rooting rate, etc. The effect of reducing production costs

Inactive Publication Date: 2008-05-28
HUAZHONG UNIV OF SCI & TECH
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that utilizes yew lateral root proliferation to produce paclitaxel, this method provides a new way for paclitaxel drug source, alleviates the pain of paclitaxel on the pharmaceutical mar

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In March of that year, 1-year-old, middle-upper, sunny-growing yew branches with full leaf buds, thick branches, short internodes, and strong growth vigor were selected as cutting materials. Cut the yew branches into 8cm long stems to use as cuttings. The upper incision is smooth and the lower incision is horse-eared. Leaves and side branches are removed below 2 / 3 of the cuttings, leaving only one side branch and leaf at the upper end. Bundle 30 cuttings of the cut yew stems into a bundle, and insert 1 / 4 (about 2 cm) of the base into 0.04% KMnO 4 Soak in the solution for 8 hours, rinse with clean water; then insert the base of the cuttings into 1% sucrose solution and soak for 12 hours, then rinse with clean water; finally soak in 10mg / L α-sodium naphthalene acetate for 2 hours, and then the cuttings are soaked in 2% FeSO 4 The solution was sprayed on the sterilized sand, placed in a cool and ventilated place, and sprayed with Hoagland (Hoagland's) nutrient solution eve...

Embodiment 2

[0026] In March of that year, choose 1-year-old, middle-upper, sunny-growing branches of Southern yew with full leaf buds, thick branches, short internodes, and strong growth potential as cutting materials. Cut the yew branches into 8cm long stems to use as cuttings. The upper incision is smooth and the lower incision is horse-eared. Leaves and side branches are removed below 2 / 3 of the cuttings, leaving only one side branch and leaf at the upper end. Bundle 30 cuttings of the cut yew stems into a bundle, and insert 1 / 4 (about 2 cm) of the base into 0.04% KMnO 4 Soak in the solution for 8 hours, rinse with clean water; then insert the base of cuttings into 1% sucrose solution and soak for 12 hours, then rinse with clean water; finally soak in 500mg / L 2,4-dichlorophenoxybutyl acetate for 2 hours and cuttages in 2% FeSO 4 The solution was sprayed on the sterilized sand, placed in a cool and ventilated place, and sprayed with Hoagland (Hoagland's) nutrient solution every 3 days ...

Embodiment 3

[0028]In April of that year, choose 1-year-old, middle-upper, sunny-growing branches of Southern yew with full leaf buds, thick branches, short internodes, and strong growth potential as cutting materials. Cut the yew branches into 8cm long stems to use as cuttings. The upper incision is smooth and the lower incision is horse-eared. Leaves and side branches are removed below 2 / 3 of the cuttings, leaving only one side branch and leaf at the upper end. Bundle 30 cuttings of the cut yew stems into a bundle, and insert 1 / 4 (about 2 cm) of the base into 0.04% KMnO 4 Soak in the solution for 8 hours, rinse with clean water; then insert the base of the cuttings into 1% sucrose solution and soak for 12 hours, then rinse with clean water; finally soak in 500mg / Lα-naphthaleneacetic acid for 2 hours, and then cuttages were soaked in 2% FeSO 4 The solution was sprayed on the sterilized sand, placed in a cool and ventilated place, and sprayed with Hoagland (Hoagland's) nutrient solution ev...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method of utilizing taxus lateral root proliferation to produce paclitaxel, the steps are that: (1) annual or biennial taxus branches are selected as cutting materials; (2) after the cuttings are sequentially injected into KMnO4 solution, sucrose solution, and plant growth hormone solution for soaking, the cuttings are conveyed to cutting substrate; (3) the cuttings are pulled out after the roots grow up, and are continually conveyed to the cutting substrate after being processed with hormone; (4) a certain quantity of the lateral roots are cut for producing the paclitaxel; (5) being dried under temperature control at the temperature that is less than or equal to 55 DEG C, pure products of the paclitaxel are obtained after processes of grinding, concentrated organic solvent extraction, column chromatography, solvent recovery, crystallizing and drying; or the cut taxus lateral roots are cultured in a improved Murashige and amp;Skoog fluid nutrient medium, bacteriostat is added in the nutrient medium, 2 to 9 days after fresh taxus lateral roots are induced and processed by elicitors, the pure products of the paclitaxel are extracted. The invention provides a new approach for medicine sources of the paclitaxel, and the shortcomings in artificial cultivation of the taxus are solved of low rooting rate, low survival rate after the roots are cut, slow roots proliferation speed, etc.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology, biopharmaceuticals, forest product chemistry and industry, and specifically relates to a method for producing paclitaxel by multiplying lateral roots of yew. Background technique [0002] Paclitaxel is currently an important anti-tumor drug recognized internationally, and it is also the only natural compound that can promote tubulin polymerization and inhibit its depolymerization. Due to its unique anti-tumor mechanism and significant anti-tumor activity, paclitaxel ranks first in the market share of anti-tumor drugs. [0003] Currently, paclitaxel used in research and clinical trials is mostly extracted from the bark of natural yew plants. The paclitaxel content in the bark is very small, only 0.007-0.01% of the dry weight of the bark, and usually 15-30 tons of bark will be used for every 1 kg of pure paclitaxel extracted. According to calculations, six yew trees with an age of 60-10...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/00A01G7/00A01G31/00C07D305/14
Inventor 余龙江付春华李丽琴邓慧夏娟
Owner HUAZHONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap