Alfalfa drought tolerance identification method

An identification method and a technology of drought tolerance, applied in the field of plant physiology, can solve the problems of evaluation research, inconsistency, and evaluation method inconsistency, and achieve the effect of strong operability and exclusion of influence

Inactive Publication Date: 2016-03-16
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many and inconsistent evaluation methods for alfalfa drought resistance, and t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for identifying drought tolerance of alfalfa, comprising the steps of:

[0024] S1. Select alfalfa seeds with the same maturity and size, remove mildew and impurities, use carbendazim spray to kill the small sclerotia and hyphae attached to the surface of the seeds, rinse with distilled water for 3 times, and perform a conventional germination experiment;

[0025] S2, when 3 true leaves appear in the seedlings, carry out thinning and final seedlings, keep the plant size, the material with the same height, then enter the greenhouse to start the water control test, and carry out the real-time measurement of the moisture in the agar bed by the moisture automatic measurement system;

[0026] S3, on the 8th day, take out and count the root quantity of each alfalfa, including tap root and lateral root and the fibrous root quantity on the lateral root;

[0027] S4, after processing 20d, measure the seedling plant height, fresh root system weight, root system dry weigh...

Embodiment 2

[0035] S1. Select alfalfa seeds with the same maturity and size, remove mildew and impurities, use carbendazim spray to kill the small sclerotia and hyphae attached to the surface of the seeds, rinse with distilled water for 3 times, and perform a conventional germination experiment;

[0036] S2, when 4 true leaves appear in the seedlings, carry out thinning and fixed seedlings, keep the plant size, the material with the same height, then enter the greenhouse to start the water control test, and carry out the real-time measurement of the moisture in the agar bed by the moisture automatic measurement system;

[0037] S3, on the 11th day, take out and check the root quantity of each alfalfa, including tap root and lateral root and the fibrous root quantity on the lateral root;

[0038] S4, after processing 20d, measure the seedling plant height, fresh root system weight, root system dry weight, leaf chlorophyll content, stomatal conductance, leaf free water content, leaf surface ...

Embodiment 3

[0046] S1. Select alfalfa seeds with the same maturity and size, remove mildew and impurities, use carbendazim spray to kill the small sclerotia and hyphae attached to the surface of the seeds, rinse with distilled water for 3 times, and perform a conventional germination experiment;

[0047] S2, when 4 true leaves appear in the seedlings, carry out thinning and fixed seedlings, keep the plant size, the material with the same height, then enter the greenhouse to start the water control test, and carry out the real-time measurement of the moisture in the agar bed by the moisture automatic measurement system;

[0048] S3, on the 10th day, take out and count the root quantity of each alfalfa, including tap root and lateral root and the fibrous root quantity on the lateral root;

[0049] S4, after processing 20d, measure the seedling plant height, fresh root system weight, root system dry weight, leaf chlorophyll content, stomatal conductance, leaf free water content, leaf surface ...

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Abstract

The invention discloses an alfalfa drought tolerance identification method, comprising the following steps: after sterilization on seeds, carrying out a conventional sprouting experiment, after a seedling has 3-4 main leaves, performing thinning and final singling, reserving materials whose plant size and height are roughly consistent, and carrying out a water controlling experiment in a greenhouse; in the 8th-11th day, taking the plants out to count the number of roots of each plant of alfalfa, including the number of main roots, lateral roots, and fibrils on the lateral roots; after processing for 20 d, respectively measuring seedling height, fresh weight of root systems, dry weight of root systems, leaf chlorophyll contents, stomatal conductance, leaf free water content, leaf surface bound water content, proline content and root volume of each processing group; performing data reduction, calculating average values, and calculating a drought tolerance coefficients and a comprehensive drought tolerance value of each variety; and determining salt tolerance of the varieties according to the comprehensive drought tolerance value. The method performs multivariate statistical analysis on agronomical characters and indexes of an alfalfa seedling stage, so as to perform comprehensive judgement and evaluation on the drought tolerance of alfalfa.

Description

technical field [0001] The invention relates to the field of plant physiology, in particular to a method for identifying drought tolerance of alfalfa. Background technique [0002] Alfalfa is an excellent leguminous forage with a wide range of planting, but in some areas, drought limits its growth rate and yield. Therefore, the principle of water use of alfalfa has been a research hotspot. Liu Guoli et al. studied the response of water use efficiency of alfalfa to water stress, and the results showed that water stress can improve its water use efficiency, but there are differences among varieties. The drought resistance of alfalfa is affected by many factors. The identification of its drought resistance requires not only appropriate indicators, but also the establishment of a scientific quantitative system on this basis. The currently recognized evaluation methods include direct comparison method, total drought resistance evaluation method, and membership function. method,...

Claims

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Application Information

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IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 刘香萍李国良杨智明曲善民
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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