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Medium for aseptic sowing and rapid seedling of single-flower Paphiopedilum

A technology of aseptic sowing and culture medium, applied in the fields of botanical equipment and methods, horticulture, gardening tools/equipment, etc., can solve problems such as large differences between species, poor seedling growth, and difficulty in germination of Paphiopedilum, to shorten the cycle, The effect of improving production efficiency

Inactive Publication Date: 2017-05-10
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] International research on Paphiopedilum plants started relatively late. Compared with other species of orchids, it is more difficult to germinate when aseptically sown, and there are large differences between species and poor seedling growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0013] The preferred embodiment of the medium for aseptic seeding and rapid seedling formation of Paphiopedilum singularis of the present invention is:

[0014] include:

[0015] Seeding medium: 1 / 4MS+tryptone 2g / L+sucrose 20g / L+agar 5.8g / L, pH=6;

[0016] Subsequent culture medium: Huabao 1#2g / L+banana 10g / L+mashed potatoes 100g / L+sucrose 20g / L+activated carbon 1g / L+agar 5.8g / L, pH=6;

[0017] Strong seedling medium: Huabao 1#2g / L+NAA0.1mg / L+6-BA0.2mg / L+banana 10g / L+mashed potatoes 100g / L+sucrose 20g / L+activated carbon 2g / L+agar 5.8g / L, pH = 6.

[0018] The single-flowered Paphiopedilum includes the native species and hybrid species of the Paphiopedilum species of the subgenus Parvisepalum, the subgenus Brachypetalum, the subgenus Paphiopedilum, and the subgenus Barbata.

[0019] Take the mature fruit pod period to cultivate: when the fruit pod is not cracked and the stalk of the fruit pod begins to turn yellow.

[0020] The medium for aseptic seeding and rapid seedling formation of pap...

specific Embodiment

[0023] Harvest period of mature pods: when the pods are not cracked and the stalks of the pods begin to turn yellow.

[0024] Seeding medium: 1 / 4MS+tryptone 2g / L+sucrose 20g / L+agar 5.8g / L, pH=6;

[0025] Subsequent culture medium: Huabao 1#2g / L+banana 10g / L+mashed potatoes 100g / L+sucrose 20g / L+activated carbon 1g / L+agar 5.8g / L, pH=6;

[0026] Strong seedling medium: Huabao 1#2g / L+NAA0.1mg / L+6-BA0.2mg / L+banana 10g / L+mashed potatoes 100g / L+sucrose 20g / L+activated carbon 2g / L+agar 5.8g / L, pH = 6.

[0027] The specific implementation includes steps:

[0028] (1) The fruit pods used for aseptic sowing are more effective when they are just ripe but not yet cracked, and the fruit pods start to turn yellow when the stalks. At this time, the seeds in the capsule can be separated from each other, making it easy to sow. Immature seeds are stuck together when sowing, which is not conducive to later growth. In addition, it is easy to sterilize the unopened pods, and only the epidermis of the pod...

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PUM

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Abstract

The invention discloses a medium for aseptic sowing and rapid seedling of single-flower Paphiopedilum. The medium comprises a sowing medium, a subculture medium and a seedling-strengthening medium, wherein the sowing medium is composed of 1 / 4MS, tryptone with a concentration of 2 g / L, cane sugar with a concentration of 20 g / L and agar with a concentration of 5.8 g / L and has a pH value of 6; the subculture medium is composed of Huabao No. 1 with a concentration of 2 g / L, banana with a concentration of 10 g / L, smashed potato with a concentration of 100 g / L, cane sugar with a concentration of 20 g / L, active carbon with a concentration of 1 g / L and agar with a concentration of 5.8 g / L and has a pH value of 6; and the seedling-strengthening medium is composed of Huabao No. 1 with a concentration of 2 g / L, NAA with a concentration of 0.1 mg / L, 6-BA with a concentration of 0.2 mg / L, banana with a concentration of 10 g / L, smashed potato with a concentration of 100 g / L, cane sugar with a concentration of 20 g / L, active carbon with a concentration of 2 g / L and agar with a concentration of 5.8 g / L and has a pH value of 6. A harvesting period for mature capsules is that the capsules do not crack and the stem parts of the capsules begin to become yellow. The medium provided by the invention allows time for aseptic sowing and rapid seedling of Paphiopedilum to be shortened to 8 months from 12 to 15 months in the prior art, so the periods of aseptic sowing and rapid seedling are greatly shortened, and production efficiency is improved.

Description

Technical field [0001] The invention relates to a flower culture medium, in particular to a culture medium for aseptic seeding and rapid seedling formation of single-flowered paphiopedilum. Background technique [0002] Paphiopedilum, also known as slipper orchid, cinderella, etc., has a very high ornamental value due to its unique flower type, gorgeous flower color, and long-lasting flowering period. It is a very popular high-end flower in the international flower market and has a high Economic Value. However, due to the devastating mining of wild resources of Paphiopedilum, Paphiopedilum has become one of the endangered species in the world, and all wild species are listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Trading is prohibited. [0003] The research on Paphiopedilum in the world started relatively late. Compared with other species of orchids, the germination of Paphiopedilum is more difficult when asep...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 郑宝强王雁
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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