Strain of Tulasnella calospora QS104 and application and method for promoting growth of paphiopedilumm aseptic seedlings

A technology of QS104 and gelatinous bacteria, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of low survival rate of transplanting, restriction of artificial breeding of Paphiopedilum, and slow growth of aseptic seedlings And other issues

Pending Publication Date: 2019-11-05
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the aseptic sowing and large-scale propagation techniques of many Paphiopedilum species have been successful, but the growth of aseptic seedlings is slow and the survival rate of transplanting is low, which restricts the artificial breeding of Paphiopedilum

Method used

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  • Strain of Tulasnella calospora QS104 and application and method for promoting growth of paphiopedilumm aseptic seedlings
  • Strain of Tulasnella calospora QS104 and application and method for promoting growth of paphiopedilumm aseptic seedlings
  • Strain of Tulasnella calospora QS104 and application and method for promoting growth of paphiopedilumm aseptic seedlings

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Experimental program
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Effect test

Embodiment 1

[0048] 1. Observation and isolation of mycorrhizal fungi

[0049] Material: P. armeniacum (P. armeniacum) is a terrestrial or semi-epiphytic plant, produced in western Yunnan (Bijiang, Hushui). Born in limestone soil at an altitude of 1400-2100 meters or on rocky and well-drained grass slopes, flowering period is February-April.

[0050] Laser confocal observation of the root structure to determine the root material: WGA-AF (wheat germ agglutinin-alexa fluor conjugate) 488 (Molecular Probes) can specifically stain the chitin of the hyphae, and the root material was fixed in absolute ethanol for more than 24 hours , cut into thin slices of about 1 mm by hand, placed in 10% KOH solution overnight, and placed the material in the dye solution (10mg / ml WGA-AF 488, 0.02% Tween20 dissolved in 1×PBS (137mM NaCl, 27mM KCl, 100mM Na2HPO4, 2mM K2HPO4, pH 7.4) stained for 2h, vacuumed once every 15min during the staining period, then the material was placed in 1 × PBS solution and rinsed...

Embodiment 2

[0101] Example 2 Co-cultivation of Coomenophora mesorium QS104 and Paphiopedilum apricot aseptic seedlings

[0102] 1. Materials and methods:

[0103] The tested seedlings of Paphiopedilum apricot were 9-month-old uniform-sized sterile seedlings (plant height 2-3cm, leaves 4-5, roots 2-4, root length about 1cm). The tested strain is an endophytic fungus isolated from P. mesoporium QS104 (preservation number CGMCC NO.17777).

[0104] The co-cultivation medium was DE medium.

[0105] DE medium formula: CaCl 2 1.0mmol / L, MgSO 4 0.5mmol / L, K 2 SO 4 1.0mmol / L, KH 2 PO 4 0.4mmol / L, FeSO 4 100μmol / L, H 3 BO 4 25μmol / L, MnCl 2 33μmol / L, ZnSO 4 2.8 μmol / L, NaMoO 4 1.0 μmol / L, Na 2 EDTA 140μmol / L, yeast extract 1.0g / L, agar 8.5g / L, pH 6.0.

[0106] Co-cultivation method: insert Paphiopedilum seedlings into orchid tissue culture bottles filled with culture medium (volume 600mL, bottle mouth inner diameter 4cm, culture medium 120mL / bottle) in a sterile operating tabl...

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Abstract

The invention provides a strain of Tulasnella calospora QS104 and its application and a method for promoting growth of paphiopedilumm, and relates to the technical field of cultivation of paphiopedilum aseptic seedlings. The Tulasnella calospora QS104 was preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO. 17777. The Tulasnella calospora QS104 is a plant endophytic fungus isolated from the root of paphiopedilum, and is identified as Tulasnella calospora. The research of the invention shows that co-cultivation of the Tulasnella calospora QS104 and paphiopedilum aseptic seedlings can significantly improve the amount of dry matter of paphiopedilum, and increase contents of elements such as P, S, Mg, Fe, Zn and Mn in the paphiopedilum aseptic seedlings.

Description

technical field [0001] The invention relates to the technical field of cultivating Paphiopedilum aseptic seedlings, in particular to a strain of P. mesorium QS104, its application and a method for promoting the growth of Paphiopedilum aseptic seedlings. Background technique [0002] Paphiopedium, also known as slipper orchid, is called cinderella in Taiwan, my country. There are more than 70 species of this genus in the world, and they are widely distributed in tropical and subtropical forests from tropical Asia to Pacific islands. Paphiopedilum is a unique group of Cattleya, and it is also the most peculiar ornamental orchid. Its ornamental value is mainly reflected in (1) the flower shape is large and peculiar, and the lips are like a purse, which has auspicious and festive meaning; (2) the flower color is gorgeous and rich, covering red, white, green, yellow and other colors; (3) a There are different species blooming throughout the year, concentrated in winter and sprin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01H4/00C12R1/645
CPCA01H4/001A01H4/008C12N1/14C12N1/205C12R2001/01
Inventor 黄家林张石宝朱鑫敏秦姣胡虹
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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