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Method for tissue culture and rapid propagation of high-quality paphiopedilum maudiae seedlings

A tissue culturing and paphioplum-like technology, which is applied in the field of plant biology, can solve the problems of large separation of offspring, difficult tissue culturing, and inability to maintain, and achieves the effect of high application value and practical technology.

Active Publication Date: 2016-04-20
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because Paphiopedilum is a hybrid species, the offspring of aseptic sowing have a large separation, and it is impossible to maintain the excellent traits of the parents to obtain seedlings with uniform traits, which seriously restricts its large-scale production.
At the same time, due to the difficulty in decontamination of the explants and the slow proliferation rate during tissue culture of Paphiopediphyllum, its tissue culture is extremely difficult. A Report on Rapid Propagation of Orchid Tissue Culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Tissue culture of Paphiopedilum SCBGMiracle

[0016] (1) Material selection and processing

[0017] Choose Paphiopedilum SCBGMiracle with strong plant growth potential and round flowers as the mother plant. 35 days before cutting the explants (side buds) for disinfection, irrigate the leaves once with a 500-fold diluted aqueous solution of carbendazim and spray the leaves. After 5 days, irrigate them once with a 500-fold diluted aqueous solution of 35% gram soil bacteria wettable powder. Spray the leaves, and after another 5 days, use a 3000-fold diluted aqueous solution of 72% agricultural streptomycin soluble powder to water once and spray the leaves. Then every 5 days, repeat the above steps, and 5 days after the last watering of agricultural streptomycin, use a scalpel sterilized with a 75% alcohol aqueous solution to cut a 2.5 cm long lateral bud as an explant.

[0018] (2) Disinfection of explants

[0019] On the ultra-clean workbench, the cut explants were s...

Embodiment 2

[0026] Example 2: Tissue culture of Paphiopedilum SCBGYunzhijun

[0027] (1) Material selection and processing

[0028] Choose Paphiopedilum SCBGYunzhijun with strong plant growth potential and round flowers as the mother plant. 35 days before cutting the explants (side buds) for disinfection, irrigate the leaves once with a 600-fold diluted aqueous solution of carbendazim and spray the leaves, 5 days later, irrigate once with a 600-fold diluted aqueous solution of 35% gram soil bacteria wettable powder Spray the leaves, and after another 5 days, irrigate the leaves once with a 3500 times diluted aqueous solution of 72% agricultural streptomycin soluble powder. Then every 5 days, repeat the above steps, 5 days after the last watering of agricultural streptomycin, use a scalpel sterilized with a 75% alcohol aqueous solution to cut a 2.5 cm long lateral bud as an explant.

[0029] (2) Disinfection of explants

[0030] On the ultra-clean workbench, the cut explants were soaked in 75% a...

Embodiment 3

[0037] Example 3: Tissue culture of PaphiopedilumSCBGRedJewel

[0038] (1) Material selection and processing

[0039] Choose Paphiopedilum (PaphiopedilumSCBGRedJewel) with strong growth potential and round flowers as the mother plant. 35 days before the explants (side buds) were cut and disinfected, the leaves were irrigated and sprayed with an 800-fold diluted aqueous solution of carbendazim. After 5 days, they were irrigated with an 800-fold diluted aqueous solution of 35% gram soil bacteria wettable powder. Spray the leaves, and after another 5 days, water once with a 4000-fold diluted aqueous solution of 72% agricultural streptomycin soluble powder and spray the leaves. Then every 5 days, repeat the above steps, and 5 days after the last watering of agricultural streptomycin, use a scalpel sterilized with a 75% alcohol aqueous solution to cut a 1.5 cm long lateral bud as an explant.

[0040] (2) Disinfection of explants

[0041] On the ultra-clean workbench, the cut explants are...

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PUM

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Abstract

The invention discloses a method for tissue culture and rapid propagation of high-quality paphiopedilum maudiae seedlings. According to the method, after a series of pharmaceutical treatment is conducted on paphiopedilum maudiae stock plants, adventitious bud induction, clump shoot regeneration and rooting culture are conducted with newly grown lateral buds as the explants by means of a unique culture medium, and then rapid propagation of high-quality seedlings is achieved. An effective means is provided to meet the market requirement for high-quality paphiopedilum maudiae seedlings. The technique is practicable, and application value is high.

Description

Technical field: [0001] The invention belongs to the field of plant biotechnology, and specifically relates to a method for rapid propagation of tissue culture of high-quality paphiopedilum species seedlings. Background technique: [0002] Paphiopedilum Maudiae is a hybrid between P.lawrenceanum and P.callosum. Using it as the parent, many hybrid offspring have been bred. The type of paphiopedilum that has a flower shape similar to that of Paphiopedilum is collectively referred to as "P.Maudiaetype" in the market. Their flowers and colors are very rich. It ranges from purple-black, red-brown to green-white, and its spots and lines are also very rich. It is the main variety of Paphiopedilum in the international and domestic markets. The conventional propagation of Paphiopedilum Modi can use ramets, but the slow propagation speed and low propagation rate are far from meeting the needs of the commercial market. At present, the Morti Paphiopedilum sold in the world market is produc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008A01H4/001
Inventor 曾宋君罗白雪江南傅艳燕周慧君吴坤林张建霞段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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