Solid-state freeze-dried product of quantitative strain and preparation method and using method thereof

Abstract

The invention discloses a solid-state freeze-dried product of a quantitative strain and a preparation method and a using method thereof. 0.1% to 0.5% of glucose, 5% to 10% of sucrose, 0.5% to 1.5% of gelatin, 1% to 2% of tryptone, 0.5% to 1% of soy peptone, 0.5% to 1% of sodium chloride, 0.2% to 0.5% of yeast powder and 0.2% to 0.5% of activated carbon are prepared into solution serving as a protective agent, and after the solution and diluted bacteria suspension are uniformly mixed according to a certain proportion, the mixture is prepared into the dried product by freezing-drying; the product is excellent in solubility and can be completely dissolved in a dissolving agent in 1 to 2 seconds. The cylindrical freeze-dried product has an amount of bacteria of 100cfu to 1,000cfu or can customize bacterium content in a specific range. One single freeze-dried product is taken and dissolved into N ml of dissolving agent, and the bacterium content per N tenths ml is smaller than 100cfu, so that the solid-state freeze-dried product is accurate and convenient. Use of the protective agent can greatly reduce a damage degree of the strain in the freeze-drying process; the proper protective agent provides an excellent shape and freeze-drying environment for the product so as to guarantee activity of the strain to be protected to the greatest degree. The dissolving agent provides an excellent pH value, an excellent osmotic pressure and proper nutrient substances to the strain so as to guarantee the strain to obtain the optimal recovery conditions after the strain is dissolved.

Description

Technical field [0001] The invention relates to a solid freeze-dried substance of quantitative bacterial strains, and a preparation method and a use method thereof, which are applicable but not limited to preparing test strains that meet the requirements of the biological inspection method in the Chinese Pharmacopoeia. Background technique [0002] The inoculation material inoculated in the freeze-dried product of the present invention is generally a single or multiple microorganisms, such as a certain strain used in the inspection of drugs, food, cosmetics, and medical devices. Therefore, the lyophilized product prepared by the present invention can be used in many fields, especially in the fields of medicine, food, cosmetics, etc., applicable but not limited to biological inspection method, sensitivity test, sterility inspection, antibacterial efficacy inspection, quantitative and qualitative control , Microbiological inspection quality control, etc. [0003] In order to better ...

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