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Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof

A respiratory syndrome, heat-resistant freeze-drying technology, applied in the field of heat-resistant freeze-drying protection agent and preparation of porcine reproductive and respiratory syndrome live vaccine, can solve problems such as preservation under -15 ℃ conditions, achieve high aging resistance, The effect of low virus loss rate, convenient storage and transportation

Active Publication Date: 2011-08-31
WUHAN CHOPPER BIOLOGY
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AI Technical Summary

Problems solved by technology

Mix the protective agent with porcine reproductive and respiratory syndrome virus antigen, and freeze-dry according to a certain freeze-drying curve. Live vaccines need to be stored at -15°C

Method used

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  • Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with implementation examples:

[0010] 1. Preparation of vaccine antigen

[0011] The virus seed used for production is the highly pathogenic porcine reproductive and respiratory syndrome virus JXA1-R strain, which is identified, kept and provided by the China Animal Disease Control Center. Select the required number of cells (cell spinner flasks that have grown into a monolayer), discard the growth medium, inoculate the production virus seed virus at 1% (V / V), and add DMEM cell culture medium containing 2% fetal bovine serum , 37°C rotating (9-12 revolutions / hour) culture. Observe the CPE caused by the virus under a microscope. When the CPE reaches more than 70%, harvest the cell culture, freeze and thaw once, remove cell debris by centrifugation or filtration, and freeze and store below -40°C. According to the appendix of "Chinese Veterinary Pharmacopoeia", the vaccine antigens shall be tested for ste...

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Abstract

The invention relates to a porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and a preparation method thereof. The heat-resistant freeze-drying protective agent is mixed by the raw materials based on weight percentage: 1-2% of gelatin, 4-6% of trehalose, 1-2% of tryptone, 1-2% of trypticase, 1-2% of polyvinylpyrrolidone, 0.164% of dipotassium phosphate, 0.052% of monopotassium phosphate, 1% of sorbic alcohol, 1% of sodium glutamate, 1% of arginine salt and the balance of water. The preparation method comprises the steps of: preparing the materials into two mixed solution, respectively preparing in a mixing way by means of degerming to prepare a heat-resistant freeze-drying protective agent, mixing according to the proportion of the protective agent to the virus-resistant raw material as 1:1, performing split charging, and drying in a freezing way. Before drying in a freezing way, the virus loss rate is low, a freeze-drying product is high in ageing-resistant degree, and the virus is invariable in content after the freeze-drying product is preserved at the temperature of 2-8 DGE C for 24 months. After the invention is used, the technical neck bottle that the porcine reproductive and respiratory syndrome live vaccine can be preserved and transported under the condition of -15 DEG C is solved.

Description

Technical field [0001] The invention belongs to the technical field of agricultural veterinary biology, and relates to freeze-drying technology of biological products, in particular to a heat-resistant freeze-dried protective agent for porcine reproductive and respiratory syndrome live vaccines and a preparation method. Background technique [0002] At present, attenuated live vaccines are one of the main vaccines for preventing infectious diseases in livestock and poultry. Live vaccines account for a large proportion of animal vaccines in my country. The protective agents commonly used by domestic vaccine companies are mainly sucrose, milk, gelatin, etc., with simple formulations and poor protection performance. If it is stored at 2~8℃, the storage period is only 3~6 months, and it usually needs to be stored below -15℃. However, the development of traditional domestic animal vaccine protective agents is relatively lagging, which greatly restricts the long-term storage and long-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K39/12A61P31/14A61K47/02A61K47/10A61K47/16A61K47/26A61K47/32
Inventor 杨雷漆世华舒银辉刘汉平祝春花温文生李伟秦洪刚肖敏
Owner WUHAN CHOPPER BIOLOGY
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