Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof

Abstract

The invention relates to a porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and a preparation method thereof. The heat-resistant freeze-drying protective agent is mixed by the raw materials based on weight percentage: 1-2% of gelatin, 4-6% of trehalose, 1-2% of tryptone, 1-2% of trypticase, 1-2% of polyvinylpyrrolidone, 0.164% of dipotassium phosphate, 0.052% of monopotassium phosphate, 1% of sorbic alcohol, 1% of sodium glutamate, 1% of arginine salt and the balance of water. The preparation method comprises the steps of: preparing the materials into two mixed solution, respectively preparing in a mixing way by means of degerming to prepare a heat-resistant freeze-drying protective agent, mixing according to the proportion of the protective agent to the virus-resistant raw material as 1:1, performing split charging, and drying in a freezing way. Before drying in a freezing way, the virus loss rate is low, a freeze-drying product is high in ageing-resistant degree, and the virus is invariable in content after the freeze-drying product is preserved at the temperature of 2-8 DGE C for 24 months. After the invention is used, the technical neck bottle that the porcine reproductive and respiratory syndrome live vaccine can be preserved and transported under the condition of -15 DEG C is solved.

Description

Technical field

[0001] The invention belongs to the technical field of agricultural veterinary biology, and relates to freeze-drying technology of biological products, in particular to a heat-resistant freeze-dried protective agent for porcine reproductive and respiratory syndrome live vaccines and a preparation method. Background technique

[0002] At present, attenuated live vaccines are one of the main vaccines for preventing infectious diseases in livestock and poultry. Live vaccines account for a large proportion of animal vaccines in my country. The protective agents commonly used by domestic vaccine companies are mainly sucrose, milk, gelatin, etc., with simple formulations and poor protection performance. If it is stored at 2~8℃, the storage period is only 3~6 months, and it usually needs to be stored below -15℃. However, the development of traditional domestic animal vaccine protective agents is relatively lagging, which greatly restricts the long-term storage and long-...

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