Bacterium and fungus strain storage culture medium

A strain preservation and culture medium technology, applied in the direction of bacteria, fungi, preservation of microorganisms, etc., can solve the problems of inability to preserve strains for a long time, complicated preparation process, short preservation time, etc., to improve the survival rate and reproduction rate, Simple to prepare, long-lasting results

Inactive Publication Date: 2012-10-10
苏桂华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have certain defectives, and as freeze-drying method, preparation process is complicated, needs better equipment condition again; However, some special strains have a low survival rate in the glycerin stock solution, and the storage time is short, and the toxicity is reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: a bacterial and fungal strain preservation medium, prepared according to the following data and described steps: tryptone 0.8-1.2g, sodium chloride 0.8-1.2g, agar (semi-solid) 0.2-0.5g, Yeast paste 0.3-0.6g, glycerol 15ml, distilled water 85ml.

[0009] The steps of the preparation method are as follows: A. Add tryptone, sodium chloride, agar (semi-solid), yeast extract and glycerol to distilled water in proportion; B. Dissolve by heating and adjust pH to 7.5; C. Dispense in EP tubes, 0.5-1ml per tube, D, 8 pounds, sterilized for 20 minutes, and stored at -20°C.

Embodiment 2

[0010] Example 2: A bacterial and fungal strain preservation medium, prepared according to the following data and described steps: tryptone 1.0 g, sodium chloride 1.0 g, agar (semi-solid) 0.3 g, yeast extract 0.5 g, glycerol 15ml, distilled water 85ml.

[0011] The steps of the preparation method are as follows: A. 1.0 g of detryptone, 1.0 g of sodium chloride, 0.3 g of agar (semi-solid), 0.5 g of yeast extract, and 15 ml of glycerol are added to distilled water; B. heated to dissolve, and adjusted to pH 7.5; C, Dispense into EP tubes, 0.5-1 ml per tube, sterilize D, 8 pounds for 20 minutes, and store at -20°C.

Embodiment 3

[0012] Example 3: A bacterial and fungal strain preservation medium, prepared according to the following data and described steps: tryptone 0.9g, sodium chloride 0.9g, agar (semi-solid) 0.4g, yeast extract 0.6g, glycerol 15ml, distilled water 85ml.

[0013] The steps of the preparation method are as follows: A. Take 0.9 g of tryptone, 0.9 g of sodium chloride, 0.4 g of agar (semi-solid), 0.6 g of yeast extract, and 15 ml of glycerol into distilled water; B. Heat to dissolve, adjust pH to 7.5; C, Dispense into EP tubes, 0.5-1 ml per tube, sterilize D, 8 pounds for 20 minutes, and store at -20°C.

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PUM

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Abstract

The invention relates to a bacterium and fungus strain storage culture medium comprising the following components: tryptone, sodium chloride, agar, yeas extract and glycerol. The tryptone is used for providing a microorganism nitrogen source; the sodium chloride is used for maintaining a uniform osmotic pressure; the agar is used as a supporting component in the culture medium; the yeas extract is rich in proteins, amino acids, peptides, nucleotides and vitamins B and can be used for replenishing various vitamins, amino acids and growth factors for the growth of microorganisms; and the glycerol is used as a carbon source and can be used for playing roles of preventing freeze and preserving moisture. The bacterium and fungus strain storage culture medium is simple and convenient in operation of strain storage, long in storage time, high in stability and capable of increasing the survival rate and the reproductive rate of the strains.

Description

technical field [0001] The invention relates to the technical field of preservation of bacterial species, in particular to a bacterial and fungal bacterial species preservation medium. Background technique [0002] At present, the commonly used strain preservation methods include freeze-drying method, semi-solid medium method and simple glycerol preservation method. These methods all have certain defects, such as freeze-drying method, the preparation process is complicated, and requires better equipment conditions; semi-solid medium preservation method cannot preserve bacteria for a long time; pure glycerol preservation method, the glycerol is only for antifreeze and moisturizing, However, some special strains have low survival rate in glycerol stock solution, short storage time and reduced toxicity. SUMMARY OF THE INVENTION [0003] Aiming at the deficiencies of the prior art, the present invention provides a bacterial and fungal culture storage medium with simple operat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/04
Inventor 苏桂华崔海玲王金环王海鹏
Owner 苏桂华
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