Method of using wheat straws for producing bacterium cellulose

A technology of bacterial cellulose and wheat straw is applied in the field of preparation of bacterial cellulose, which can solve the problems of complex structure of wheat straw, and achieve the effect of low price and wide source.

Inactive Publication Date: 2009-09-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main chemical components of wheat straw are composed of cellulose, hemicellulose and lignin, but the structure of wheat straw is complex

Method used

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  • Method of using wheat straws for producing bacterium cellulose

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Effect test

Embodiment 1

[0034] Wheat straw is first ground with a plant pulverizer, then soaked overnight (12-24h) with dilute sulfuric acid (3%, w / v) in a reactor at a solid / liquid ratio of 1:6 between the straw and dilute acid solution, Then react at a temperature of 121° C. for 60 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0035] Add NaOH to adjust the pH value of the hydrolyzed liquid to about 10, filter the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 30°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute...

Embodiment 2

[0037] Wheat straw is first ground with a plant grinder, and then soaked overnight (12-24h) in a reactor with dilute hydrochloric acid (1%, w / v) at a solid / liquid ratio of 1:10 straw to dilute acid solution, Then react at a temperature of 121° C. for 75 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0038] Add Ca(OH) 2 Adjust the pH value of the hydrolyzed liquid to about 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 40°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute ...

Embodiment 3

[0040] Wheat straw is first ground with a plant pulverizer, and then soaked overnight (12-24h) with dilute sulfuric acid (2%, w / v) in a reactor with a solid / liquid ratio of 1:15 straw to dilute acid solution, Then react at a temperature of 100° C. for 80 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0041] Add 25% ammonia water to adjust the pH value of the hydrolyzed liquid to 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and fine-tune the pH value to 10. Then seal it with a film, place it in a 25°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute s...

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Abstract

The invention relates to a method of using wheat straws for producing bacterium cellulose, which includes the following steps: wheat straws are milled into 40 to 80 meshes and immersed by dilute sulphuric acid or hydrochloric acid in a reaction container according to the solid/liquid ratio of 1:6 to 1:30 of the wheat straws to the dilute acid liquid, the wheat straws react with the dilute sulphuric acid or hydrochloric acid at the temperature of 100 DEG C to 121 DEG C, the residual slag of the wheat straws and the acid hydrolyzed liquid are separated by sucking filtration, and the hydrolyzed liquid is collected and refrigerated for spare; the hydrolyzed liquid is detoxified; the detoxified hydrolyzed liquid is used as the carbon source of culture medium, 0.1 percent to 1 percent of yeast concrete and 0.1 percent to 0.5 percent of tryptone are added to be prepared into culture medium, the acetobacter or the glucose oxidized bacilli is vaccinated with the vaccinating amount of 6 percent to 10 percent to be cultivated in an oscillating table of 36 to 30 DEG C and 160 to 250 r/m or statically cultivated in an incubator of 26 DEG C to 30 DEG C, and the bacterium cellulose is obtained. The carbon source of culture medium prepared by the method has good quality and low price, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for producing bacterial cellulose by utilizing wheat straw. Background technique [0002] Bacterial Cellulose (BC for short), also known as Microbial Cellulose, is a biological material with broad application prospects. Compared with other higher plant cellulose in nature, it has many unique properties. Including high purity, high crystallinity, high degree of polymerization, high water holding capacity, high tensile strength, strong biological adaptability, etc. Therefore, the cellulose material has great application prospects in the fields of artificial skin and blood vessels, medical dressings, adhesives, vibration membranes for audio equipment, papermaking, textiles, and composite membranes. However, the high cost of bacterial cellulose culture medium, low cellulose yield and yield are the bottlenecks of its industrial production and popularization and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/01
Inventor 洪枫朱颖雪郭建平杜明
Owner DONGHUA UNIV
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