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Nematode-eating fungus with nematode-killing function, preparing method and use thereof

A nematode-eating fungus, a nematode-killing technology, applied in the field of nematode-eating fungi and its preparation, capable of solving problems such as groundwater pollution

Inactive Publication Date: 2005-09-28
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Large doses of chemical pesticides have seriously polluted groundwater

Method used

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  • Nematode-eating fungus with nematode-killing function, preparing method and use thereof
  • Nematode-eating fungus with nematode-killing function, preparing method and use thereof
  • Nematode-eating fungus with nematode-killing function, preparing method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The mycelia of Dactylella shizishanna, YMF1.00022 were inoculated on flat agar medium, and the medium formula was PDA medium formula. The mycelium of Dactylella shizishanna, YMF1.00022 was inoculated on the culture medium, cultured at 28° C. for 10-14 days, and a plate seed was obtained.

[0045]Plate seed is inoculated in 250ml Erlenmeyer flask liquid medium (60ml of every bottle), and liquid medium formula is: 0.15% gelatin; 0.8% tryptone; 0.1% yeast extract powder; 0.05% ammonium sulfate; 0.001% ferrous sulfate; 0.05% magnesium sulfate; 1.129% disodium hydrogen phosphate; 1.238% sodium dihydrogen phosphate pH6.5, the rest is water. The culture time was 7 days on a shaker at 27° C., and the rotation speed was 220 rpm.

[0046] Filter the fermentation culture with clean gauze to remove the mycelium, take the supernatant, slowly add ammonium sulfate at a ratio of 1:0.56, let stand at 4°C for 2 hours, then centrifuge at 8500rpm for 15 minutes, and discard the supernatan...

Embodiment 2

[0048] It is basically the same as in Example 1, except that the formula of the liquid medium is: 0.1% gelatin; 0.6% tryptone; 0.15% yeast extract powder.

Embodiment 3

[0050] It is basically the same as Example 1, except that the formula of the liquid medium is: 0.2% gelatin; 0.7% tryptone; 0.2% yeast extract powder.

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Abstract

The present invention is one kind of nematode eating fungi capable of killing nematode and its preparation process and application, and belongs to the field of microbial pesticide technology. The nematode eating fungus is obtained through liquid fermentation of the strain, extracting the effective nematode killing component from the metabolic product and subsequent preparation step. The nematode eating fungus strain is Dactylella shizishanna YMF1.00022, and the liquid fermentation culture medium consists of gelatin, tryptone, yeast leaching powder, ammonium sulfate, ferrous sulfate, magnesium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and water in certain proportion. The extracting process of the effective nematode killing component includes taking supernatant from the fermented matter, adding ammonium sulfate, centrifuging, dissolving in buffering phosphoric acid solution, dialysis and other steps.

Description

Technical field: [0001] The invention relates to a nematode-eating fungus with the function of killing nematodes and its preparation method and application, belonging to the technical field of microbial pesticides. Background technique: [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known types of root-knot nematodes alone, which damage more than 3,000 plants and cause huge losses every year. According to statistics from the Food and Agriculture Organization of the United Nations, nematodes cause global losses of up to 100 billion U.S. dollars every year. Nematodes in my country mainly harm tobacco, flowers, vegetables, cotton, soybeans, peanuts, Panax notoginseng, American ginseng and other major economic crops, and become an important limiting factor for the development of these crops. For a long time, nematode control has mainly relied on chemical control. Although chemical nematicides have played an important role in nem...

Claims

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Application Information

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IPC IPC(8): A01N63/04C12N1/14C12N1/16
Inventor 张克勤王瑞宾周薇
Owner YUNNAN UNIV
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