Kit for fluorescent PCR (Polymerase Chain Reaction) detection of high-risk HPV (Human Papilloma Virus)

Abstract

The invention provides a kit for fluorescent PCR (Polymerase Chain Reaction) detection of a high-risk HPV (Human Papilloma Virus). The kit comprises a nucleic acid releasing agent and a PCR reaction solution, wherein the nucleic acid releasing agent comprises 0.01-05mmol / L surfactin, 20-300mmol / L potassium chloride, 0.01-2% sodium dodecyl sulfate and 0.05-1% ethanol; the PCR reaction solution comprises high-risk HPV16-type and 18-type primer probe sequences and internal standard primer probe sequences. The invention aims at providing a kit for fluorescent quantitative PCR detection of high-risk Human Papilloma Virus, and the kit has the advantages of fastness in operation, simple method, high detection sensitivity and wide detection range. By virtue of the kit, a common fast fluorescent PCR detection is carried out on DNA nucleic acid fragments of the high-risk human papilloma virus in unknown samples, such as cast-off cells of the surface of a verruca acuminata body, women cervical epithelial cells and genital secretion and the detection results can be applied in the auxiliary diagnosis of the high-risk human papilloma virus infection and the early screening of cervical cancer.

Description

technical field

[0001] The invention provides a high-risk fluorescent PCR detection kit for human papillomavirus (HPV). Background technique

[0002] Human papillomavirus (HPV) is a kind of non-enveloped double-stranded circular DNA virus with small molecular weight, which obligately infects and parasitizes the epithelial cells of human reproductive organs and other tissues and organs. Clinically, HPV can be divided into high-risk and low-risk types according to the different pathogenicity or cancer risk of different HPV subtypes. Low-risk HPV mainly causes exophytic wart-like lesions on the anus skin, male external genitalia, female labia, urethral meatus, lower vagina, and low-grade cervical intraepithelial neoplasia. High-risk HPV can not only cause external genital warts, but more importantly, external genital cancer, cervical cancer and high-grade cervical intraepithelial neoplasia. The virus subtypes mainly include HPV16, 18, 31, 33, 35, 39, and 45 , 51, 52, 53, 56, ...

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