Human papilloma virus infection gene amplification fluorescent detection kit

Abstract

The invention discloses a probe and a primer which have nucleotide sequence complementary with HPV DNA, wherein, the probe is selected from SEQ ID Nos: 1 to 13; the primer is selected from SEQ ID SEQ ID Nos: 14 to 39; the invention also discloses a fluorescence identification kit comprising the probe and the primer and used for indentifying nucleic acid amplification infected by high-risk human papillomavirus, which contains 13 varieties of high-risk human papillomavirus subtypes and can finish extracting and detecting 80 to 100 portions of clinical samples DNA in 3 hours. The invention has the advantages of high sensitivity and specificity and low price, thus being easy to be accepted by patients and easy for popularizing and spreading.

Description

technical field [0001] The invention relates to a probe and a primer with a nucleotide sequence complementary to human papilloma virus HPV DNA, and a fluorescent detection kit for gene amplification of human papilloma virus infection containing the probe and primer. Background technique [0002] Human papillomavirus (HPV) is closely related to cervical cancer. HPV can be divided into high-risk and low-risk types. It has been clinically proven that 95-99.7% of cervical cancer cases are related to high-risk HPV infection. HPV infection causes cervical cancer The relative risk of cancer increases 250-fold. The existence of HPV indicates the existence of the disease. Early diagnosis of HPV infection is of great significance to clear the persistent infection of the female reproductive tract and prevent it from evolving into cervical cancer. [0003] Traditionally, cervical smear detection is a routine method for population screening, but because this method only uses observation...

AI Technical Summary

Problems solved by technology

In the past, although conventional PCR technology was used to detect HPV infection quickly and easily, there were two major defects: ① In the process of post-processing the amplified product, if the operation is not careful, it is easy to contaminate and cause false positive

② It can only be qualitative, not quantitative, and cannot observe the relationship between HPV load and cervical lesions, thus limiting its application in clinical work

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