Human papilloma virus infection gene amplification fluorescent detection kit

A fluorescent labeling and base technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problems of inability to observe the relationship of HPV load, easy contamination of false positives, and inability to quantify, etc., to achieve Easy to popularize, high sensitivity and specificity, easy to accept the effect

Active Publication Date: 2009-07-22
潮州凯普生物化学有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, although conventional PCR technology was used to detect HPV infection quickly and easily, there were two major defects: ① In the process of post-processing the amplified product, if the operation is not careful, it is easy to contaminate and cause false positive
② It can only be qualitative, not quantitative, and cannot observe the relationship between HPV load and cervical lesions, thus limiting its application in clinical work

Method used

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  • Human papilloma virus infection gene amplification fluorescent detection kit
  • Human papilloma virus infection gene amplification fluorescent detection kit
  • Human papilloma virus infection gene amplification fluorescent detection kit

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Embodiment Construction

[0027] The present invention will be described in detail below through examples.

[0028] The present inventor prepared DNA complementary to 13 high-risk HPVs (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68) respectively by analyzing the DNA of clinical samples. The probes of SEQ ID Nos: 1-13 and the primers of SEQ ID Nos: 14-39 are used to detect HPV infection and identify HPV high-risk types.

[0029] The nucleic acid amplification (PCR) fluorescent identification kit of high-risk human papillomavirus (HPV) infection provided by the present invention includes the above-mentioned probes and primers, DNA polymerase, and also includes PCR MIX, which includes all PCR reactions except the template Required reagents, as well as HPV53 plasmid for positive control and ultrapure water for negative control.

[0030] The steps of using the kit of the present invention are as follows:

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Abstract

The invention discloses a probe and a primer which have nucleotide sequence complementary with HPV DNA, wherein, the probe is selected from SEQ ID Nos: 1 to 13; the primer is selected from SEQ ID SEQ ID Nos: 14 to 39; the invention also discloses a fluorescence identification kit comprising the probe and the primer and used for indentifying nucleic acid amplification infected by high-risk human papillomavirus, which contains 13 varieties of high-risk human papillomavirus subtypes and can finish extracting and detecting 80 to 100 portions of clinical samples DNA in 3 hours. The invention has the advantages of high sensitivity and specificity and low price, thus being easy to be accepted by patients and easy for popularizing and spreading.

Description

technical field [0001] The invention relates to a probe and a primer with a nucleotide sequence complementary to human papilloma virus HPV DNA, and a fluorescent detection kit for gene amplification of human papilloma virus infection containing the probe and primer. Background technique [0002] Human papillomavirus (HPV) is closely related to cervical cancer. HPV can be divided into high-risk and low-risk types. It has been clinically proven that 95-99.7% of cervical cancer cases are related to high-risk HPV infection. HPV infection causes cervical cancer The relative risk of cancer increases 250-fold. The existence of HPV indicates the existence of the disease. Early diagnosis of HPV infection is of great significance to clear the persistent infection of the female reproductive tract and prevent it from evolving into cervical cancer. [0003] Traditionally, cervical smear detection is a routine method for population screening, but because this method only uses observation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 谢龙旭
Owner 潮州凯普生物化学有限公司
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