HPV (human papilloma virus) high-risk typing fluorescence PCR (polymerase chain reaction) detection kit

Abstract

The invention provides an HPV (human papilloma virus) high-risk typing fluorescence PCR (polymerase chain reaction) detection kit which comprises a nucleic acid releaser and two or more PCR reaction solutions, wherein the nucleic acid releaser comprises 0.01-0.5 mmol / L surfactin, 20-300 mmol / L potassium chloride, 0.01-2% of sodium dodecylsulfate and 0.05-1% of ethanol. One of the PCR reaction solutions comprises high-risk HPV 16 type and / or 18 type primer probe sequences, and another PCR reaction solution comprises an internal reference primer probe sequence. The kit provided by can be used for carrying out quick fluorescence PCR typing detection on DNA (deoxyribonucleic acid) nucleic acid segments of high-risk HPV in wart surface shedding cells, female cervix epithelial cells, genital secretion and other unknown samples; and the detection result can be used for auxiliary diagnosis of high-risk HPV infection, early screening of cervical carcinoma, follow-up survey of cervix pathological changes and instruction of vaccine development.

Description

technical field

[0001] The invention provides a high-risk type typing fluorescent PCR detection kit for human papillomavirus (HPV). Background technique

[0002] Human papillomavirus (HPV) is a kind of non-enveloped double-stranded circular DNA virus with small molecular weight, which obligately infects and parasitizes the epithelial cells of human reproductive organs and other tissues and organs. Clinically, HPV can be divided into high-risk and low-risk types according to the different pathogenicity or cancer risk of different HPV subtypes. Low-risk HPV mainly causes exophytic wart-like lesions on the anus skin, male external genitalia, female labia, urethral meatus, lower vagina, and low-grade cervical intraepithelial neoplasia. High-risk HPV can not only cause external genital warts, but more importantly, external genital cancer, cervical cancer and high-grade cervical intraepithelial neoplasia. The virus subtypes mainly include HPV16, 18, 31, 33, 35, 39, and 45 , 51, ...

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