Application of polypeptide in preparing preparations for preventing and treating human papilloma virus infection

A technology of human papillomavirus and preparation, which is applied in the field of polypeptides, and can solve the problem of no inhibition of HPV activity

Pending Publication Date: 2020-07-07
GENLOCI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The applicant discovered the polypeptide described in the present invention in the research. The polypeptide is an antimicrobial peptide. There is no report of its ability to inhibit HPV activity in the prior art. The applicant has tested that the polypeptide has a significant inhibitory effect on HPV infection

Method used

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  • Application of polypeptide in preparing preparations for preventing and treating human papilloma virus infection
  • Application of polypeptide in preparing preparations for preventing and treating human papilloma virus infection
  • Application of polypeptide in preparing preparations for preventing and treating human papilloma virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 polypeptide vaginal gel

[0032] (1) The formula is as follows:

[0033] The polypeptide 0.04%

[0034] Hydroxypropyl Methyl Cellulose 2%

[0035]Ethylhexylglycerin 0.3%

[0036] Purified water balance

[0037] (2) Process flow

[0038] Swell the hydroxypropyl methylcellulose, reheat and redissolve it into a solution-like substrate, add the polypeptide and ethylhexylglycerin to the substrate in sequence at 80 degrees, stir and mix well, and adjust the pH value to 5.5. Made of polypeptide vaginal gel.

Embodiment 2

[0039] The preparation of embodiment 2 polypeptide cleaning solution

[0040] (1) The formula is as follows:

[0041] The polypeptide 0.2%

[0042] Glycerin 0.5%

[0043] Ethylparaben 0.02%

[0044] Ethanol 0.32%

[0045] Arginine 0.6%

[0046] Lysine 5%

[0047] Purified water balance

[0048] (2) Process flow

[0049] Dissolve ethylparaben in ethanol for use, premix and stir glycerin for use, mix the polypeptide, arginine, and lysine in purified water, add to the glycerin solution in the previous step, and then add nipagin The gold ethyl ester solution was stirred evenly, and the pH value was adjusted to 6.2 to prepare the polypeptide cleaning solution.

Embodiment 3

[0050] The protein interaction relationship between polypeptide described in embodiment 3 and HPV antigen

[0051] The capsid of HPV is composed of structural proteins L1 and L2. L1 alone or L1 and L2 can be assembled into virus-like particles after co-expression. L1 protein is highly conserved in all types of HPV and participates in the packaging of HPV complete virus particles and the interaction between HPV and the host Binding of cell receptors.

[0052] BIA (Biomolecular Interaction Analysis) is a biosensing analysis technology based on a physical optical phenomenon of surface plasmon resonance (SPR). BIA can provide real-time observation of the interaction between biomolecules, through which the specificity of the binding of two molecules can be observed. It can know how strong the combination of two molecules is, and how many synergists and participants there are in the combination process of biomolecules.

[0053] In this experiment, BIA technology will be used to obs...

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Abstract

The invention discloses an application of polypeptide in preparing preparations for preventing and treating human papilloma virus infection, and belongs to the field of infectious disease treatment. Apolypeptide preparation for preventing and treating human papilloma virus infection is also provided; and the polypeptide preparation takes the polypeptide with effective dose as active ingredients and is prepared by adding pharmaceutically acceptable subsidiary materials or auxiliary components. The polypeptide can be used for the treatment and prevention of human papilloma virus infection, so that the polypeptide is significant in effect; and the polypeptide has no inhibition effects on the normal flora of female genital tracts, so that the polypeptide is good in safety.

Description

technical field [0001] The invention relates to a novel antiviral polypeptide in the technical field of medicine, in particular to the use of a polypeptide for preventing and controlling human papillomavirus infection. Background technique [0002] Human papillomavirus (HPV) belongs to the papillomaviridae family (papillomaviridae). It is a kind of epitheliophilic non-enveloped double-stranded DNA virus. ) and other symptoms. With the rapid increase in the incidence of genital warts in sexually transmitted diseases and the increase in cervical cancer and anal cancer, HPV infection has attracted more and more attention. There are many subtypes of HPV. HPV isolated from human skin and mucous membranes is divided into more than 100 subtypes, and can be divided into "low-risk" type and "high-risk" type according to their carcinogenicity. Low-risk type (such as types 6 and 11) are mainly related to genital warts and low-grade cervical epithelial necrosis, and representatives of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P31/20
CPCA61K38/10A61P31/20A61K38/00A61K38/04A61K9/0036A61K9/06A61K47/38A61K47/10A61K47/183A61K9/02
Inventor 凌建群王益行唐启慧
Owner GENLOCI BIOTECH
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