Photosynthetic bacterium immobilizing process

A technology of photosynthetic bacteria and purpose, applied in the field of immobilization of photosynthetic bacteria, to achieve the effect of not easy to run off, keep water quality stable, and easy to transport

Inactive Publication Date: 2006-08-02
刘军义 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using zeolite powder as the main material to make the immobilized carrier of photosynthetic bacteria has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Preparation of zeolite powder: directly adopt commercial zeolite powder with a particle size of 120 mesh.

[0014] 2. Preparation of the photosynthetic bacteria concentrate: flocculate the cells of the photosynthetic bacteria culture solution overnight with a calcium hydrogen phosphate solution with a concentration of 5 g / L, and absorb the supernatant by siphoning to obtain the photosynthetic bacteria concentrate.

[0015] 3. Cell immobilization: take 50kg of zeolite powder, add 2kg of sodium alginate with a viscosity of 500mpa s, mix well, then add 12.5kg of photosynthetic bacteria concentrate, mix well, and use a granulator to form granules with a particle size of About 5mm, the formed particles are placed in CaCl with a concentration of 20g / L 2 Harden in the solution for 12 hours; after hardening, take it out and rinse it twice with tap water, put it in a plastic bag, and seal it.

[0016] 4. Product preservation: use room temperature preservation.

[0017] 5. P...

Embodiment 2

[0019] 1. Preparation of zeolite powder: directly adopt commercial zeolite powder with a particle size of 200 mesh.

[0020] 2, the preparation of photosynthetic bacteria concentrate: with embodiment 1.

[0021] 3. Bacteria immobilization: Take 100kg of zeolite powder, add 3kg of sodium alginate with a viscosity of 1000mpa s, mix well, then add 20kg of photosynthetic bacteria concentrate, mix well, and use a granulator to form granules with a particle size of 5mm Left and right, the formed particles are placed in CaCl with a concentration of 40g / L 2 Harden in the solution for 1 hour. After hardening, take it out and rinse it twice with tap water, put it in a plastic bag, and seal it.

[0022] 4. Product preservation and application are the same as in Example 1.

Embodiment 3

[0024] 1. Preparation of zeolite powder: directly adopt commercial zeolite powder with a particle size of 150 mesh.

[0025] 2. Preparation of the photosynthetic bacteria concentrate: flocculate the cells of the photosynthetic bacteria culture solution overnight with a calcium hydrogen phosphate solution with a concentration of 2 g / L, and absorb the supernatant by siphoning to obtain the photosynthetic bacteria concentrate.

[0026] 3. Cell immobilization: Take 100kg of zeolite powder, add 5kg of sodium alginate with a viscosity of 800mpa s, mix well, then add 30kg of photosynthetic bacteria concentrate, mix well, and use a granulator to form granules with a particle size of About 5mm, the formed particles are placed in CaCl with a concentration of 30g / L 2 Harden in the solution for 6 hours; after hardening, take it out and rinse it twice with tap water, put it in a plastic bag, and seal it.

[0027] 4. Product preservation and application are the same as in Example 1.

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Abstract

The photosynthetic bacterium immobilizing process includes preparing zeolite powder of 120-200 mesh, adding sodium alginate solution of 500-1000 mpa.s viscosity and concentrated photosynthetic bacterium liquid in the weight ratio of 1:0.03 to 0.05:0.2-0.3, mixing through stirring, pelletizing, hardening in 20-40 g / L concentration CaCl2 solution, and final clear water rinsing to obtain the product. The process has great photosynthetic bacterium immobilizing amount, high immobilizing efficiency, and high activity of the immobilized photosynthetic bacterium, and the product can deposit fast to water bottom for degrading organic pollutant and purifying water without loss.

Description

technical field [0001] The invention belongs to the field of immobilization method of photosynthetic bacteria. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria, referred to as PSB) is a large class of bacteria that can carry out non-oxygenous photosynthesis. Ninth edition) can be divided into 6 taxa and 27 genera. The six taxa are Chromobacteriaceae, Exothorhodospiraceae, Purple Non-sulfur Bacteria, Green Sulfur Bacteria, Multicellular Chlorothycium, Halobacteria, etc. Because it has the physiological characteristics of using small organic molecules such as ammonia nitrogen and organic acids as growth factors, and the bacteria itself is non-toxic, harmless and nutritious, it has been widely used in the aquaculture industry. [0003] There have been many reports on the application of photosynthetic bacteria in aquaculture, but most of them are researched on liquid cultures, that is, free photosynthetic bacteria. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00C12N11/14C12N11/04
Inventor 刘军义汪文龙邓润祯
Owner 刘军义
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