Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating

A technology of solid-phase microextraction head and nucleic acid aptamer, which is applied in the direction of chemical instruments and methods, other chemical processes, material separation, etc., can solve the problems of interaction, easily polluted environment, restrictions, etc., and achieve enhanced enrichment efficiency, The effect of overcoming the low loading rate and increasing the loading capacity

Inactive Publication Date: 2013-04-24
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

However, due to the limitations of molecular imprinting technology itself, there are still the following shortcomings: the rigid recognition "cavity" in molecularly imprinted polymer (MIP) is easily damaged or deformed; MIP coating is relatively limited in the analysis of biological samples; its Specificity and affinity are not as good as biological recognition systems such as enzyme-substrate, antibody-receptor interactions; most of the extraction solvents are organic solvents, which are e

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  • Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating
  • Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating
  • Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating

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Embodiment Construction

[0035] This embodiment takes adenosine as an example to describe the present invention in detail, but this does not limit the protection scope of the present invention.

[0036] Such as figure 1 Shown, the preparation method based on nucleic acid aptamer / graphene oxide / porous polymer coating SPME extraction tip is as follows:

[0037] (1) Soak 5.0cm long quartz fiber in acetone for 0.5h, take it out, and wash it with ethanol three times; then soak it in benzene for 0.5h, take it out, wash it with ethanol three times, and then wash it with distilled water three times; put it in 1.0mol / L NaOH alkaline washing solution at room temperature for 1 hour, take it out, and wash it three times with distilled water; after alkali washing, the quartz fiber is immediately soaked in 1.0mol / L HCl washing solution for 1 hour, take it out, and wash it with distilled water. After alkali washing and acid washing, the quartz fiber was activated in an oven at 150°C for 1 hour. Take out the quart...

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Abstract

The invention discloses a preparation method for a novel solid-phase micro-extraction head based on a single-stranded DNA aptamer modified graphene oxide coating. The preparation method comprises the following steps: (1) subjecting a quartz fiber to silanization; (2) coating a porous polymer solution on the surface of the quartz fiber; (3) repeatedly coating a porous polymer coating; (4) carrying out silanization; (5) intercalating the silanized porous polymer coating quartz fiber into a mixed solution of a PBS buffer solution, a catalyst EDC solution, an NHS solution and an aqueous graphene oxide solution; (6) repeating step (4) and step (5) a plurality of times on the same quartz fiber; and (7) taking out the graphene oxide/porous polymer coating quartz fiber and intercalating the graphene oxide/porous polymer coating quartz fiber into a mixed solution of the PBS buffer solution, the EDC solution, the NHS solution and an aptamer solution. The preparation method provided by the invention overcomes the disadvantages of a low immobilization rate, easy shedding, instability and the like of conventional coating methods, substantially increases the immobilization rate of an aptamer and improves enrichment efficiency of SPME.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing instruments, and relates to a method for preparing an extraction head based on a single-stranded DNA nucleic acid aptamer / graphene oxide / porous polymer solid-phase microextraction coating. It is suitable for high selectivity, rapid separation and enrichment of trace alkaloids, antibiotics or nucleotides in complex biological samples. Background technique [0002] Sample pretreatment methods and techniques have always been one of the important topics and development directions in the field of modern chemistry. A complete sample analysis process includes sample collection, sample pretreatment, analysis and determination, data processing and reporting results, among which the time required for sample pretreatment accounts for about 60% of the entire analysis time, and may bring more than 30% of the time Therefore, the error has become the bottleneck problem in the analysis work, and it i...

Claims

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Application Information

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IPC IPC(8): B01J20/282B01J20/32G01N30/08
Inventor 胡小刚范亚男蔡泉林伍莉莉柳晓飞
Owner SOUTH CHINA NORMAL UNIVERSITY
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