Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating

Abstract

The invention discloses a preparation method for a novel solid-phase micro-extraction head based on a single-stranded DNA aptamer modified graphene oxide coating. The preparation method comprises the following steps: (1) subjecting a quartz fiber to silanization; (2) coating a porous polymer solution on the surface of the quartz fiber; (3) repeatedly coating a porous polymer coating; (4) carrying out silanization; (5) intercalating the silanized porous polymer coating quartz fiber into a mixed solution of a PBS buffer solution, a catalyst EDC solution, an NHS solution and an aqueous graphene oxide solution; (6) repeating step (4) and step (5) a plurality of times on the same quartz fiber; and (7) taking out the graphene oxide / porous polymer coating quartz fiber and intercalating the graphene oxide / porous polymer coating quartz fiber into a mixed solution of the PBS buffer solution, the EDC solution, the NHS solution and an aptamer solution. The preparation method provided by the invention overcomes the disadvantages of a low immobilization rate, easy shedding, instability and the like of conventional coating methods, substantially increases the immobilization rate of an aptamer and improves enrichment efficiency of SPME.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing instruments, and relates to a method for preparing an extraction head based on a single-stranded DNA nucleic acid aptamer / graphene oxide / porous polymer solid-phase microextraction coating. It is suitable for high selectivity, rapid separation and enrichment of trace alkaloids, antibiotics or nucleotides in complex biological samples. Background technique [0002] Sample pretreatment methods and techniques have always been one of the important topics and development directions in the field of modern chemistry. A complete sample analysis process includes sample collection, sample pretreatment, analysis and determination, data processing and reporting results, among which the time required for sample pretreatment accounts for about 60% of the entire analysis time, and may bring more than 30% of the time Therefore, the error has become the bottleneck problem in the analysis work, and it i...

AI Technical Summary

Problems solved by technology

However, due to the limitations of molecular imprinting technology itself, there are still the following shortcomings: the rigid recognition "cavity" in molecularly imprinted polymer (MIP) is easily damaged or deformed; MIP coating is relatively limited in the analysis of biological samples; its Specificity and affinity are not as good as biological recognition systems such as enzyme-substrate, antibody-receptor interactions; most of the extraction solvents are organic solvents, which are easy to pollute the environment

[0007] Compared with the extensive research and application of graphene oxide and nucleic acid aptamers in their respective related fields, the combination of graphene oxide and nucleic acid aptamers is applied to solid-phase microextraction technology, and graphene oxide is used as a carrier to provide large Specific surface area, using nucleic acid aptamer as the recognition unit to improve selectivity, there is no relevant literature report at home and abroad

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