Method for optimizing activated sludge population structure and enriching ammonia oxidation bacteria
A technology of ammonia oxidizing bacteria and activated sludge, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of increasing investment costs and adaptability of strains, and achieve energy saving and high enrichment efficiency effect
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Embodiment 1
[0033] Taking the actual domestic sewage discharged from a family area of a university as the test object (pH=7.8-8.0, COD=260-350mg / L, TN=80-90mg / L,), C / N is 3.3-4.3, which belongs to low carbon-nitrogen ratio sewage. The selected SBR reactor has an effective volume of 10L, and the COD concentration of the mixed liquid in the reactor is maintained at 200-300mg / L, and the NH 4 + -N concentration is about 45mg / L, and the initial MLSS in the reactor is 3.05~3.5.0g.L -1 , maintain the DO value at about 1.5mg / L, maintain the sludge age at about 8d, and maintain the reaction temperature at 25°C. By running the real-time control system for 61 days, stable short-cut nitrification was realized, and the nitrite accumulation rate reached over 95%. Molecular biology FISH test showed that the elimination of NOB could be achieved through continuous sludge discharge at the end of aeration, making AOB the dominant bacterial group in the nitrification process.
Embodiment 2
[0035] The test water is domestic sewage in the family area of the school. Raw water COD is 120.8~259.7mg / L, NH 4 + -N concentration is 44~65mg / L, BOD 5 105~150mg / L, NO 3 - -N concentration is 0.04~0.42mg / L, NO 2 - -N concentration is 0.06~0.15mg / L, C / N is 2.72~4.0, which is low carbon nitrogen ratio and low ammonia nitrogen sewage. The effective volume of the SBR reactor is 14L, and the COD concentration of the mixed liquid in the reactor is maintained at 100-200mg / L, NH 4 + -N concentration is about 40mg / L, and the initial MLSS in the reactor is 3.0g.L -1 About 1.8mg / L or so, maintain the DO value at about 1.8mg / L, maintain the sludge age at about 5d, and keep the reaction temperature at 30°C. By running the real-time control system for 32 days, stable short-cut nitrification was realized, and the nitrite accumulation rate reached over 95%. Fluorescence in situ hybridization (FISH) detection showed that the content of AOB in the sludge increased by 58.9%, and the...
Embodiment 3
[0037] Taking the effluent from the primary sedimentation tank of a city sewage treatment plant as the test object, the COD of the raw water is 308.8-440.7mg / L, and the NH 4 + -N concentration is 44.1~55.3mg / L, BOD 5 155.8~250.3mg / L, NO 3 - -N concentration is 0.04~0.12mg / L, NO 2 - -N concentration is 0.06-0.10mg / L, C / N is 7-8. The selected SBR reactor has an effective volume of 6L, and the COD concentration of the mixed liquid in the reactor is maintained at 200-300mg / L, and the NH 4 + -N concentration is 45mg / L, the initial MLSS in the reactor is 2.05~2.50g.L -1 , maintain the DO value at about 2.0mg / L, maintain the sludge age at about 10d, and maintain the reaction temperature at 26.5°C. By running the real-time control system for 60 days, stable short-range nitrification has been realized, the nitrite accumulation rate has reached over 95%, and the TN removal rate has reached over 95%. Molecular biology FISH detection showed that AOB became the dominant bacterial ...
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