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Method for optimizing activated sludge population structure and enriching ammonia oxidation bacteria

A technology of ammonia oxidizing bacteria and activated sludge, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of increasing investment costs and adaptability of strains, and achieve energy saving and high enrichment efficiency effect

Active Publication Date: 2009-04-15
HEILONGJIANG BISHUIYUAN ENVIRONMENTAL PROTECTION ENG CO LTD
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  • Abstract
  • Description
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Problems solved by technology

For engineering applications, if inoculated with purely cultured strains, it will face increased investment costs and problems with the adaptability of the strains to water quality

Method used

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  • Method for optimizing activated sludge population structure and enriching ammonia oxidation bacteria

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Experimental program
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Effect test

Embodiment 1

[0033] Taking the actual domestic sewage discharged from a family area of ​​a university as the test object (pH=7.8-8.0, COD=260-350mg / L, TN=80-90mg / L,), C / N is 3.3-4.3, which belongs to low carbon-nitrogen ratio sewage. The selected SBR reactor has an effective volume of 10L, and the COD concentration of the mixed liquid in the reactor is maintained at 200-300mg / L, and the NH 4 + -N concentration is about 45mg / L, and the initial MLSS in the reactor is 3.05~3.5.0g.L -1 , maintain the DO value at about 1.5mg / L, maintain the sludge age at about 8d, and maintain the reaction temperature at 25°C. By running the real-time control system for 61 days, stable short-cut nitrification was realized, and the nitrite accumulation rate reached over 95%. Molecular biology FISH test showed that the elimination of NOB could be achieved through continuous sludge discharge at the end of aeration, making AOB the dominant bacterial group in the nitrification process.

Embodiment 2

[0035] The test water is domestic sewage in the family area of ​​the school. Raw water COD is 120.8~259.7mg / L, NH 4 + -N concentration is 44~65mg / L, BOD 5 105~150mg / L, NO 3 - -N concentration is 0.04~0.42mg / L, NO 2 - -N concentration is 0.06~0.15mg / L, C / N is 2.72~4.0, which is low carbon nitrogen ratio and low ammonia nitrogen sewage. The effective volume of the SBR reactor is 14L, and the COD concentration of the mixed liquid in the reactor is maintained at 100-200mg / L, NH 4 + -N concentration is about 40mg / L, and the initial MLSS in the reactor is 3.0g.L -1 About 1.8mg / L or so, maintain the DO value at about 1.8mg / L, maintain the sludge age at about 5d, and keep the reaction temperature at 30°C. By running the real-time control system for 32 days, stable short-cut nitrification was realized, and the nitrite accumulation rate reached over 95%. Fluorescence in situ hybridization (FISH) detection showed that the content of AOB in the sludge increased by 58.9%, and the...

Embodiment 3

[0037] Taking the effluent from the primary sedimentation tank of a city sewage treatment plant as the test object, the COD of the raw water is 308.8-440.7mg / L, and the NH 4 + -N concentration is 44.1~55.3mg / L, BOD 5 155.8~250.3mg / L, NO 3 - -N concentration is 0.04~0.12mg / L, NO 2 - -N concentration is 0.06-0.10mg / L, C / N is 7-8. The selected SBR reactor has an effective volume of 6L, and the COD concentration of the mixed liquid in the reactor is maintained at 200-300mg / L, and the NH 4 + -N concentration is 45mg / L, the initial MLSS in the reactor is 2.05~2.50g.L -1 , maintain the DO value at about 2.0mg / L, maintain the sludge age at about 10d, and maintain the reaction temperature at 26.5°C. By running the real-time control system for 60 days, stable short-range nitrification has been realized, the nitrite accumulation rate has reached over 95%, and the TN removal rate has reached over 95%. Molecular biology FISH detection showed that AOB became the dominant bacterial ...

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Abstract

The invention discloses an optimizing method of enriched ammonia-oxidizing bacteria in an activated sludge population structure, belongs to the technical field of enhanced biological sewage denitrification processing, aims at solving the practical problems of trenchant running condition requirements, long cultivation time of enriched ammonia-oxidizing bacteria and high running energy consumption in current denitrifying techniques with short-course nitration and denitrification, and develops the optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure according to the growth characteristics of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an activated sludge processing system. The optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure is mainly characterized in that a nitration reaction process is controlled with parameters of pH value and DO, aeration is halted before ammonia peak valley appears, ammonia-oxidizing bacteria is completely proliferated, proliferation chance of NOB is deprived, and nitrite-oxidizing bacteria is eliminated from the system by controlling sludge age, thus achieving the goal for the quick start of short-course nitration and denitrification. A bisection method is utilized in the operating process so as to determine the optimal time for advancing aeration halt. Furthermore, the optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure has the advantages of short reaction period, high enrichment efficiency, simple operation and the like.

Description

technical field [0001] The technical field of the claimed technical solution is: the technical field of sewage enhanced biological denitrification treatment, which relates to a method for cultivating ammonia oxidizing bacteria in an enhanced biological denitrification system, especially the application of short-cut nitrification and denitrification technology to the biological denitrification process system The rapid start-up and enrichment culture of ammonia oxidizing bacteria are suitable for the treatment of nitrogenous industrial wastewater and the advanced treatment of urban sewage. Background technique [0002] In order to prevent eutrophication of lakes and other receiving water bodies, all urban sewage treatment plants need to remove nitrogen and phosphorus, and biological methods have become the leading application technology. Biological nitrification and denitrification are the most commonly used biological nitrogen removal methods. It is divided into two stages: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 彭永臻李凌云刘洋马宁平王淑莹
Owner HEILONGJIANG BISHUIYUAN ENVIRONMENTAL PROTECTION ENG CO LTD
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