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Method for producing dihydroxyacetone by using gluconobacter sp.

A technology of dihydroxyacetone and Gluconobacter, which is applied in the field of dihydroxyacetone produced by Gluconobacter CGMCC No.5146, which can solve the problems of prolonged production cycle, high separation cost, complicated process, etc., to reduce solvent consumption and environmental pollution, The effect of simplifying the process and shortening the process time

Active Publication Date: 2013-09-11
中科医药行业生产力促进中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, in the production of DHA by the biological method, there is an inhibitory effect of the substrate and the product on the production strain, which leads to a decrease in the conversion rate when the concentration of glycerin increases, and prolongs the production cycle, as well as the complicated procedures in the subsequent separation and extraction of DHA produced by the fermentation method. Small separation capacity, high separation cost and low product yield, therefore, DHA has not yet achieved industrial production in China

Method used

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  • Method for producing dihydroxyacetone by using gluconobacter sp.
  • Method for producing dihydroxyacetone by using gluconobacter sp.

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Embodiment 1

[0040] Gluconobacter sp. CGMCC No.5146 was cultured for initial fermentation using sorbitol as a substrate medium, the initial concentration of sorbitol was 30g / L, the fermentation temperature was 28°C, and pH 6.0. The bacteria begin to grow slowly. After the continuous fermentation reaches a stable period, continuous fermentation is carried out in the membrane bioreactor with the inner pressure membrane of the hollow fiber ultrafiltration membrane using glycerol as the substrate. During the continuous fermentation, the membrane bioreactor continuously draws out The product DHA without bacterial cells is supplemented with fresh medium at the same time, which reduces the inhibition of the fermentation process by the accumulation of high-concentration substrates in the fermenter, and makes the accumulation of DHA take place in a longer period of time. At the same time, the bacteria were not drawn out during the whole fermentation process, but the DHA product was still accumulated...

Embodiment 2

[0045] Gluconobacter sp. CGMCC No.5146 was cultured for initial fermentation using sorbitol as a substrate medium, the initial concentration of sorbitol was 30g / L, the fermentation temperature was 28°C, and pH 6.0. The bacteria begin to grow slowly. After the continuous fermentation reaches a stable period, continuous fermentation is carried out in the membrane bioreactor with the inner pressure membrane of the hollow fiber ultrafiltration membrane using glycerol as the substrate. During the continuous fermentation, the membrane bioreactor continuously draws out The product DHA without bacterial cells is supplemented with fresh medium at the same time, which reduces the inhibition of the fermentation process by the accumulation of high-concentration substrates in the fermenter, and makes the accumulation of DHA take place in a longer period of time. At the same time, the bacteria were not drawn out during the whole fermentation process, but the DHA product was still accumulated...

Embodiment 3

[0050] The other steps and conditions are the same, and 3 times of absolute ethanol is added during the purification of DHA to remove impurities. The conversion rate of glycerol reaches more than 90%, and the yield reaches more than 80%.

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Abstract

The invention provides a method for producing dihydroxyacetone by using gluconobacter sp. CGMCC No. 5146. The method comprises the following steps of: performing initial fermentation culture by using a culture medium using sorbitol as a substrate till the thalli of the gluconobacter sp. are grown to a stable stage, putting the obtained solution containing the thalli and the dihydroxyacetone product into a membrane bioreactor, performing continuous fermentation by using a culture medium using glycerol as a substrate, continuously filtering the fermentation solution containing no thalli through the membrane bioreactor, meanwhile, constantly supplementing fresh culture medium using glycerol as the substrate to the membrane bioreactor, separating the dihydroxyacetone in the fermentation solution, and purifying the dihydroxyacetone. When the glycerol concentration of the substrate is 60g / L, the concentration of the dihydroxyacetone (DHA) is 56.75g / L, the volume yield of the DHA is 9.02 g / (L. h), the conversion rate of the glycerol reaches over 90 percent, and the yield of the DHA reaches over 80 percent; and the technical bottlenecks of low yield and high cost in a microbial fermentation method for preparing the dihydroxyacetone are broken. The method has the characteristics of simplicity and convenience in operation, energy conservation, high production efficiency and the like, and is suitable for industrialized large-scale production.

Description

technical field [0001] The invention relates to a method for producing dihydroxyacetone by Gluconobacter sp. CGMCC No.5146. Background technique [0002] Dihydroxyacetone, also known as 1,3-dihydroxyacetone, referred to as DHA, the English name is 1,3-dihydroxyacetone. DHA is the simplest ketose sugar, the apparent molecular formula is C 3 h 6 o 3 , the molecular weight is 90.07. [0003] DHA is white powder with sweet taste, water solubility >250g / L (20°C), stable at pH 6.0, melting point 70-80°C (mixture of monomer and dimer), easily soluble in water and ethanol , acetone and ether and other solvents. In general, DHA is a crystal that exists in the form of dimer (1,4-Dioxane), but it becomes a monomer after being dissolved or heated. [0004] Dihydroxyacetone (DHA) is a naturally occurring ketose sugar that is biodegradable, edible and non-toxic to humans and the environment. Containing 3 functional groups (2 hydroxyl groups, 1 carbonyl group) in the DHA molecule...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C07H3/02C07H1/06C12R1/01
Inventor 王福清徐莉张桐
Owner 中科医药行业生产力促进中心有限公司
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