A method for in vitro preservation of pig intestinal chyme microbiome

Abstract

The invention discloses an in vitro preservation method of pig intestinal chyme microbiome. The invention provides a method for cryopreserving colon microbes of growing pigs, which comprises the following steps: adding glycerin to cryopreservation of growing pig colon digesta or its inoculum, so as to realize cryopreservation of growing pig colon microbiomes. The microbiome with high similarity level preserved by this method can be directly used in fecal bacteria transplantation and dietary fiber fermentation test, which solves the problems of high cost, few donors and inconsistent standards when the same microbiome is used multiple times. It has played a positive role in promoting the production of pigs and the development of animal husbandry industry.

Description

technical field

[0001] The invention relates to the fields of biotechnology and animal husbandry, in particular to an in vitro preservation method of pig intestinal chyme microbiome. Background technique

[0002] There are a variety of microbial flora distributed on the surface of the animal body, but more microorganisms exist in the hindgut of the animal body, and the number of microorganisms in the hindgut even exceeds the total number of cells in the body. In fact, the microbiome of the hindgut can almost be regarded as an organ with complex physiological functions, which is of great significance to the health of the body. The microecological balance in the intestinal tract is a key factor in determining the health of the intestinal tract. As people's extensive use of antibiotics breaks the balance of intestinal microecology, the imbalance of intestinal microecology leads to a large number of intestinal diseases, Clostridium difficile infection (CDI) is one of the importa...

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