The invention provides a method for determining the content of
natural rubber in
rubber seed oil. The determination method has the advantages of simple instrument and equipment, simple, convenient andquick operation, low detection cost and high
accuracy and precision. The method for determining the content of the
natural rubber in the
rubber seed oil by adopting a
saponification method is characterized by comprising the following steps: firstly, precisely weighing 100 to 200 grams of
rubber seed oil sample and putting the rubber seed oil sample in a flask, adding
potassium hydroxide into theflask to form 400 to 800 milliliters of
alcohol solution, shaking up the solution and then connecting the solution to a
reflux condensing tube, boiling the solution for about 1 to 2 hours in a water bath kettle, taking down the condensing tube, filtering the saponified solution to another flask while the solution is hot, crushing up rubber grains on a piece of filter cloth, washing the rubber grains till no alkali or
soap is contained, finally kneading the rubber to form a slice, baking the slice with a vacuum oven (at the temperature of 80 DEG C) to constant weight and then weighing the slice, and calculating the content of the rubber. The rubber seed
oil can be saponified in the alkali
alcohol solution, the
natural rubber belongs to a
hydrocarbon substance and does not produce
saponification effect, and the natural rubber is coagulated into the rubber grains to be separated out during the
saponification of the rubber seed oil so as to determine the content of the natural rubber.