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Bluefin tuna collagen hydrolyzate and preparation method thereof

A technology of bluefin tuna and hydrolyzate, applied in the direction of peptide, fermentation, etc., can solve the problem that the freezing strength and antioxidant properties of tuna collagen hydrolyzate are not reported, and the collagen hydrolyzate has not been introduced, and achieves remarkable antioxidant effect. No toxic and side effects, the effect of reducing energy consumption

Active Publication Date: 2015-07-22
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the preparation method of the tuna collagen hydrolyzate, there is no introduction of the method of obtaining collagen hydrolyzate with different molecular weights by regulating the compound enzyme degreasing and deodorizing enzymatic hydrolysis extraction conditions, and the freezing strength and antioxidant properties of the tuna collagen hydrolyzate no report

Method used

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preparation example Construction

[0024] The invention provides a preparation method of a collagen hydrolyzate, which comprises the following steps: a treatment step of decalcification and impurity protein removal, a compound enzyme degreasing, deodorizing and enzymolysis extraction step, and a purification and drying step.

[0025] In the decalcification and impurity protein removal step of the present invention, the fish bones are fully drained and soaked in 0.1 to 0.2 wt% acid solution for 5 to 24 hours, preferably 7.5 to 15 hours, and the acid solution is replaced every 1.5 hours to remove the fish bones Calcium salt in the solution; use a concentration of 2~8wt%, preferably 3~5wt% sodium chloride solution to soak the fish bones to remove impurities, the soaking time is 8~14 hours, preferably 10~12 hours, and then use deionized water Wash the fish bones to remove sodium chloride.

[0026] In the degreasing and deodorizing extraction compound enzymolysis step of the present invention, the decalcified fish b...

Embodiment 1

[0033] Taking bluefin tuna fish bone as raw material to prepare collagen hydrolyzate with small molecular weight as an example, the following process steps are performed in sequence:

[0034] Step 1: Thaw the bluefin tuna fish bones, wash them with cold water, soak the fish bones in 0.1wt% hydrochloric acid solution for 7.5 hours at room temperature, replace the acid solution every 1.5 hours, and remove calcium salts; Soak fish bones in sodium chloride for 12 hours to remove miscellaneous proteins (miscellaneous proteins are non-collagen proteins);

[0035] Step 2: Clean and drain the fish bones, add deionized water at a weight ratio of fish bones to deionized water of 1:10, adjust the pH to 8, and add alkaline lipase (triacylglycerol acyl Hydrolase), according to the concentration of 2.0wt%, put into the yeast (oxidize the aldehydes in the fish bones to acid to remove the fishy smell), according to the concentration of 1.5wt%, add a kind of alkaline protease (Novo type serine...

Embodiment 2

[0040] Taking the bluefin tuna fish bone as the raw material to prepare the collagen hydrolyzate with larger molecular weight as an example, the following process steps are carried out in sequence:

[0041] Step 1: Thaw bluefin tuna fish bones, wash them with cold water, soak them in 0.2wt% citric acid solution for 15 hours at room temperature, replace the acid solution every 1.5 hours, and remove calcium salts; Soak fish bones in sodium for 6 hours to remove impurities;

[0042] Step 2: Clean and drain the fish bones, add deionized water at a weight ratio of fish bones to deionized water of 1:20, adjust the pH to 7, and add neutral lipase (triacylglycerol acyl Hydrolase), according to the concentration of 2.0wt%, put into the yeast (oxidize the aldehydes in the fish bone to acid to remove the fishy smell), according to the concentration of 1.0wt%, add a kind of papain (cysteine ​​protease), enzyme The hydrolysis temperature was adjusted to 40°C, and the enzymolysis was carri...

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Abstract

The invention relates to a bluefin tuna collagen hydrolyzate and a preparation method thereof. The method comprises the following steps: cleaning bluefin tuna bones, and immersing in an acidic solution to remove inorganic calcium salts; immersing in a sodium chloride solution to remove non-collagen impurity proteins; carrying out degreasing, fishy smell removal and enzymolysis on the treated fish bones, inactivating the enzyme at high temperature, and carrying out hydrothermal extraction to obtain a bluefin tuna collagen hydrolyzate solution; and drying to obtain the collagen hydrolyzate powder. The preparation technique is scientific and reasonable, and fully utilizes the unemployed fish bones in aquatic product processing as the raw material to develop the collagen hydrolyzate with potential market value; the composite enzyme degreasing, fishy smell removal and enzymolysis process is adopted while regulating the enzymolysis conditions of the composite enzyme, thereby greatly shortening the extraction time of the collagen hydrolyzate, preparing the tuna collagen hydrolyzate with different molecular weights and with the advantages of high gel strength, high oxidation resistance activity, high safety, no toxic or side effect and the like, enhancing the production efficiency and lowering the energy consumption.

Description

technical field [0001] The invention relates to a fish bone collagen hydrolyzate and its preparation method and application, in particular to a bluefin tuna fish bone collagen hydrolyzate and its preparation method and application. Background technique [0002] Collagen is a macromolecular protein ubiquitous in animals, and it mainly exists in the connective tissue of animals. Collagen is widely used in food, medicine, tissue engineering, cosmetics and other fields because of its good biocompatibility, bioactivity and biodegradability. Collagen hydrolyzate is a hydrolyzate obtained by hydrolyzing collagen under certain conditions. Compared with collagen, collagen hydrolyzate has a lower molecular weight, better water solubility, and is easily absorbed by the body. At the same time, collagen hydrolyzate also has more biological activities, such as: anti-oxidation, anti-aging, calcium supplementation, promoting bone development, improving immunity, and promoting skin cell re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00
Inventor 黄雅钦丁丁孟兆亭
Owner BEIJING UNIV OF CHEM TECH
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