Pseudomonas mendocina CY004 for efficiently removing nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water body and application thereof

Abstract

The invention belongs to the technical field of water environment biological treatment, in particular to a Pseudomonas mendocina strain CY004 for strongly degrade nitrite nitrogen, nitrate nitrogen and ammonia nitrogen, separation screening, safety measurement, a bacterial liquid preparation method and treatment application to domestic sewage and aquaculture sewage. The strain CY004 is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 209197. By morphology observation, physiology and biochemistry analysis and 16S rDNA homology analysis, the strain CY004 is authenticated to be Pseudomonas mendocina ymp. The strain can quickly degrade the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the water body, has no pathogenicity on animals of silver carp, sharksucker, shrimp, Eriocheir sinensis, white mouse and the like, and better degradation function on the nitrite and the ammonia nitrogen in the real sewage under normal temperature, degradation rate reaching 96.1 percent and 90 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the aquaculture sewage, degradation rate reaching 97.3 percent and 97.1 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the domestic sewage and better application prospect.

Description

technical field [0001] The invention belongs to the technical field of water environment microbial treatment, and specifically relates to the screening and application of a strain of Pseudomonas mendoza CY004 for removing nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water bodies. technical background [0002] At present, my country has done a lot of work in the prevention and control of water pollution, but the problem of eutrophication in aquaculture water bodies and natural water bodies is still very serious. The survey shows (Jin Xiangcan et al., 1995): Most of the lakes and reservoirs in my country have reached or exceeded the level of eutrophication. The "eutrophication" of the water body makes the water body smelly, fish and other organisms die in large numbers, blue-green algae bloom, and many places have no water to drink in high temperature seasons. Excessive levels of nutrients such as nitrogen and phosphorus in water bodies are the main cause of eu...

AI Technical Summary

Problems solved by technology

This has become a bottleneck restricting the research and application of traditional biological denitrification technology: on the one hand, nitrous bacteria are chemoautotrophic bacteria with a slow growth rate, resulting in a long reaction start-up time, and nitrous bacteria are sensitive to the composition, pH and temperature of sewage. Sensitive to changes (Mobarry et al, 1996)

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