Pseudomonas mendocina CY004 for efficiently removing nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water body and application thereof
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A technology of Pseudomonas spp. and nitrite nitrogen, applied in the fields of bacteria, chemical instruments and methods, biological water/sewage treatment, etc.
Inactive Publication Date: 2010-07-28
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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This has become a bottleneck restricting the research and application of traditional biological denitrification technology: on the one hand, nitrous bacteria are chemoautotrophic bacteria with a slow growth rate, resulting in a long reaction start-up time, and nitrous bacteria are sensitive to the composition, pH and temperature of sewage. Sensitive to changes (Mobarry et al, 1996)
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Embodiment 1
[0078] Embodiment 1, isolation, screening and identification of bacterial strains
[0079] 1. Isolation and screening of strains
[0080] 1. Prepare the following medium:
[0081] Denitrification medium: potassium nitrate 2.0g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 0.5g, potassium sodium tartrate 20g, distilled water 1L, pH 7.2 (adding 20g agar is a solid medium)
[0082] BTB screening medium: L-asparagine 1g, KNO 3 (or NaNO 2 )1g, KH 2 PO 4 1g, sodium succinate 8.5g, FeCl 2 .6H 2 O 0.05g, CaCl 2 .2H 2 O 0.2g, MgSO 4 .7H 2 O 0.07g, 1ml / L BTB [pH indicator bromothymol] (1%, soluble in ethanol), agar 20g, pH7.2.
[0083] 2. Screening of aerobic denitrifying bacteria
[0084] ①. Microbial selective enrichment culture: put the water sample on hold and discard the supernatant, take the activated sludge and inoculate it in a test tube with a Duchenne tubule (denitrification medium), the inoculation amount is 5%, in an incubator at 28°C Aft...
Embodiment 2
[0088] Example 2, Determination of the Ammonia Nitrogen Removal Ability of Domestic Sewage and Breeding Wastewater by Mendozamonas CY004
[0089] The domestic sewage used in the test was taken from the sewage outlet of Jingzhou Dongmen Moat, and the ammonia nitrogen concentration was measured to be 62.15mg / L, the nitrite concentration was 5.2mg / L, and pH6.0-6.8; the aquaculture wastewater was taken from the aquaculture sewage of the rice field eel breeding base of Yangtze University , the concentration of ammonia nitrogen is 5.72mg / L, the concentration of nitrite is 0.7mg / L, and the pH is 7.5-8.5. Set the concentration to 1 x 10 7 cfu / mL CY004 bacterial agent, put into the sewage according to the inoculum amount of 1‰. Incubate at room temperature for 72 hours, measure the content of ammonia nitrogen in the two kinds of sewage, and determine the denitrification ability of sewage. The specific experimental results are shown in Table 1.
[0090] Table 6 The removal effect of s...
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Abstract
The invention belongs to the technical field of water environment biological treatment, in particular to a Pseudomonas mendocina strain CY004 for strongly degrade nitrite nitrogen, nitrate nitrogen and ammonia nitrogen, separation screening, safety measurement, a bacterial liquid preparation method and treatment application to domestic sewage and aquaculture sewage. The strain CY004 is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 209197. By morphology observation, physiology and biochemistry analysis and 16S rDNA homology analysis, the strain CY004 is authenticated to be Pseudomonas mendocina ymp. The strain can quickly degrade the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the water body, has no pathogenicity on animals of silver carp, sharksucker, shrimp, Eriocheir sinensis, white mouse and the like, and better degradation function on the nitrite and the ammonia nitrogen in the real sewage under normal temperature, degradation rate reaching 96.1 percent and 90 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the aquaculture sewage, degradation rate reaching 97.3 percent and 97.1 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the domestic sewage and better application prospect.
Description
technical field [0001] The invention belongs to the technical field of water environment microbial treatment, and specifically relates to the screening and application of a strain of Pseudomonas mendoza CY004 for removing nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in water bodies. technical background [0002] At present, my country has done a lot of work in the prevention and control of water pollution, but the problem of eutrophication in aquaculture water bodies and natural water bodies is still very serious. The survey shows (Jin Xiangcan et al., 1995): Most of the lakes and reservoirs in my country have reached or exceeded the level of eutrophication. The "eutrophication" of the water body makes the water body smelly, fish and other organisms die in large numbers, blue-green algae bloom, and many places have no water to drink in high temperature seasons. Excessive levels of nutrients such as nitrogen and phosphorus in water bodies are the main cause of eu...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/38
CPCY02W10/10
Inventor 袁科平邹世平李谷何力郑卫东艾晓辉
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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